不同培养液体系对小鼠卵母细胞孤雌激活的影响

发布时间：2018-04-14 11:23

本文选题：小鼠 + 卵母细胞　；参考：《河北联合大学》2011年硕士论文

【摘要】：目的本研究以CD-1小鼠的孤雌激活胚胎和自然受精胚胎为实验材料,探讨不同的胚胎体外培养液体系对小鼠孤雌激活胚胎和小鼠自然受精胚胎体外发育的影响。目的是寻找一种合适于CD-1小鼠孤雌激活胚胎的体外培养液体系,研究同一种培养液体系对小鼠孤雌激活胚胎和小鼠自然受精胚胎的发育的影响,探讨孤雌激活胚胎的受精、卵裂、囊胚等不同发育阶段所需某种培养成分,不但为小鼠孤雌激活实验方法改进,也为人类胚胎培养方法的改善、提高人类体外受精-胚胎移植(IVF-ET)的妊娠成功率提供了实验依据。方法选择三种不同的胚胎体外培养液体系(1640、Irvine和Vitrolife)分别对小鼠孤雌激活胚胎与小鼠自然受精胚胎进行培养,观察胚胎发育情况,分析卵裂率、囊胚形成率和囊胚孵出率来比较小鼠孤雌激活胚胎与自然受精胚胎的发育率以及胚胎的不同发育阶段的影响。结果孤雌激活组1604、Irvine与Vitrolife培养液组间卵裂率(58.1%、78.5%、81.7%)、囊胚形成率(9.0%、36.5%、39.3%)、囊胚孵出率(0.6%、4.3%、7.3%)有显著差异(P0.05),三组培养液组间比较激活卵率(96.1%、94.2%、95.3%)无显著差异(P0.05);Vitrolife与Irvine培养液组间卵裂率、囊胚形成率、囊胚孵出率无显著差异(P0.05),但是孤雌激活Vitrolife培养液组胚胎的发育较孤雌激活Irvine培养液组发育好;1604培养液组卵裂率、囊胚形成率、囊胚孵出率均低于其他各组,有统计学意义(P0.05)。孤雌激活组与自然受精组之间比较激活卵率、卵裂率、囊胚形成率和囊胚孵出率无显著差异(P0.05)。结论在上述三种胚胎培养液组中:(1)Vitrolife和Irvine培养液组对小鼠孤雌激活胚胎与自然受精胚胎在卵裂率、囊胚形成率和囊胚孵出率等方面均有明显优势,并且Vitrolife培养液组结果更佳,提示Vitrolife培养液组更适合于小鼠孤雌激活实验。(2)同一种培养体系对孤雌激活胚胎与自然受精胚胎的发育的并无显著影响,也提示孤雌激活胚胎具有与自然受精胚胎相似的后续发育潜能。(3)三组培养液体系中激活卵率无显著差异,表明短时间内不同成分培养液对孤雌激活胚胎发育并无明显影响。
[Abstract]:Objective to investigate the effects of different liquid systems of embryo culture on the development of parthenogenetic activated embryos and natural fertilized embryos of CD-1 mice in vitro using parthenogenetic activated embryos and natural fertilized embryos as experimental materials.The aim of this study was to find an in vitro culture system suitable for parthenogenetic embryos in CD-1 mice, to study the effects of the same culture medium system on the development of parthenogenetic embryos and natural fertilized embryos in mice, and to explore the fertilization of parthenogenetic embryos.Some culture components needed in different stages of development, such as cleavage and blastocyst, are not only the improvement of mouse parthenogenetic activation test method, but also the improvement of human embryo culture method.Improving the pregnancy success rate of human IVF-ETS provides experimental evidence.Methods the mouse parthenogenetic activated embryos and the mouse natural fertilized embryos were cultured in three different embryo culture systems in vitro. The embryo development was observed and the cleavage rate was analyzed.The blastocyst formation rate and blastocyst hatching rate were compared to compare the developmental rate of parthenogenetic activated embryos with those of natural fertilization embryos and the effects of different stages of embryo development.Results there was significant difference in cleavage rate between 1604 Irvine group and Vitrolife culture medium group (P 0.05). There was no significant difference in blastocyst formation rate between Vitrolife group and Irvine medium group, blastocyst formation rate and blastocyst hatching rate (P 0.05). There was no significant difference in blastocyst formation rate and blastocyst formation rate between Vitrolife group and Irvine medium group.There was no significant difference in blastocyst hatching rate (P 0.05), but the blastocyst rate, blastocyst formation rate and blastocyst hatching rate in parthenogenetic activated Vitrolife medium group were significantly lower than those in parthenogenetic activated Irvine medium group.There was no significant difference between parthenogenetic activation group and natural fertilization group in activated egg rate, cleavage rate, blastocyst formation rate and blastocyst hatching rate.Conclusion in the above three kinds of embryo culture medium groups, there are obvious advantages in the cleavage rate, blastocyst formation rate and blastocyst hatching rate of mouse parthenogenetic activated embryos and natural fertilized embryos, and the results of Vitrolife culture medium group are better.The results suggest that the Vitrolife culture medium group is more suitable for mouse parthenogenetic activation test. (2) the same culture system has no significant effect on the development of parthenogenetic activated embryos and natural fertilized embryos.It was also suggested that there was no significant difference in the rate of activated eggs among the three groups, indicating that the different components of culture medium had no significant effect on the development of parthenogenetic activated embryos in a short period of time.
【学位授予单位】：河北联合大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R321

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