解氏肝癌一号方对H22荷瘤小鼠肿瘤的抑制作用研究

发布时间：2018-04-14 13:14

本文选题：原发性肝癌 + 解氏肝癌1号方　；参考：《大连医科大学》2012年硕士论文

【摘要】：目的：本文通过建立肝癌小鼠模型，给予解氏肝癌1号方干预性治疗，，通过观察不同治疗组的增殖细胞核抗原（PCNA）、胰岛素样生长因子-Ⅱ（IGF-Ⅱ）、抑瘤率、小鼠体重的相关的数据，为解氏肝癌1号方的临床应用提供理论依据。方法：无菌条件下，建立H22荷瘤小鼠模型，将小鼠随机分为盐水组、解氏肝癌1号方组、肝积方组、5氟尿嘧啶组四组，每组10只。计算抑瘤率，通过免疫组化(immunohistochemistry)的方法计算PCNA含量及双抗体夹心酶联免疫吸附法（ELISA）测定血清IGF-Ⅱ。结果：1、不同组别小鼠的抑瘤率差别有统计学意义（P＜0.05）解氏肝癌1号方组有较好的抑制肿瘤生长的作用。2、不同治疗组别小鼠的小鼠体重变化差别有统计学意义（P＜0.05）解氏肝癌1号方组的治疗可以使小鼠体重明显增加，改善小鼠的生活质量。3、不同组别间小鼠的血清IGF-Ⅱ含量差别有统计学意义（P＜0.05）解氏肝癌1号方通过降低小鼠血清IGF-Ⅱ含量抑制肿瘤。4、不同组别小鼠的PCNA指数差别有统计学意义（P＜0.05）解氏肝癌1号方通过降低小PCNA阳性表达率抑制肿瘤。结论：通过实验表明解氏肝癌1号方对小鼠的肿瘤及PCNA、IGF-Ⅱ有明显的抑制作用，抑制PCNA、IGF-Ⅱ生成可能是解氏肝癌1号方抑制肿瘤生长的作用机制之一，解氏肝癌1号方对肝癌的缩瘤方面有显著的作用。
[Abstract]:Objective: through the establishment of a mouse model for hepatocellular carcinoma, HCC No. 1, give the solution's intervention, through the observation of different treatment groups of proliferating cell nuclear antigen (PCNA), insulin-like growth factor II (IGF- II), the inhibition rate of tumor weight of mice, the relevant data, provide a theoretical basis for clinical application of liver cancer's solution No. 1.
Methods: under sterile conditions, the establishment of H22 model of tumor bearing mice, the mice were randomly divided into saline solution group, No. 1 group's liver, liver qyhj Decoction group, 5 fluorouracil group four groups, 10 rats in each group. The tumor inhibition rate was calculated by immunohistochemistry (immunohistochemistry) method to calculate the content of PCNA and double antibody sandwich enzyme-linked immunosorbent assay (ELISA) serum IGF- II.
Results: 1, the inhibition rate of mice in different groups had significant difference (P < 0.05) solution No. 1 group's liver cancer.2 better inhibition of tumor growth, significant changes of body weight in mice of different treatment group differences (P < 0.05) for treatment of hepatocellular carcinoma Xie's No. 1 group can the body weight of mice increased significantly, improve the quality of life of.3 mice, there was a significant difference between the serum levels of IGF- II content between different groups of mice (P < 0.05) solution's liver cancer No. 1, by reducing the content of IGF- in serum in inhibiting tumor of.4 mice of different groups were statistically significant difference between the PCNA index (P < 0.05) solution No. 1, by reducing the liver's tumor inhibition rate of small PCNA positive expression.
Conclusion: the experiment shows that the solution's No. 1 hepatocellular carcinoma in mice and PCNA tumor, IGF- II inhibition, inhibit PCNA, IGF- II generation may be one of the mechanisms of Xie's liver cancer No. 1, inhibit the growth of tumor, liver Xie's No. 1, has a significant effect on liver tumor shrinkage.

【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R735.7;R-332;R285.5

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