新疆维吾尔族与哈萨克族人群17个Y-STR基因座遗传多态性研究

发布时间：2018-04-14 14:27

本文选题：Y染色体 + Y-STR多态型　；参考：《新疆医科大学》2011年硕士论文

【摘要】：目的:对新疆和田地区维吾尔族与阿勒泰地区哈萨克族两民族男性Y染色体的17个Y-STR基因座的遗传多态性进行研究,揭示其遗传多态性,获得各基因座的基因频率,了解各基因座在两民族中个体识别率的差异,对Yfiler试剂盒在法医学上的应用进行探讨;同时为新疆公安机关计算和田地区维吾尔族与阿勒泰地区哈萨克族群体17个Y-STR的单倍型频率与累积个体识别率提供基础数据资料;方法:分别选取96名维吾尔族和97名哈萨克族无关男性个体血样,Chelex法提取模板DNA,选用ABI公司AmpFISTR-YfilerTM试剂盒五色荧光复合扩增检验体系:一个反应管中可同步扩增l7个Y-STR基因座;并用ABI 3130XL遗传分析仪对扩增产物进行检测分析,使用Data Collection 3.0软件进行数据收集,GeneMapper ID v3.3软件进行分析,得到Y-STR的分型结果;结果:96例维吾尔族Y-STR基因座DYS456、DYS389Ⅰ、DYS458、DYS389Ⅱ、DYS390、DYS19、DYS385a/b、DYS392、DYS391、DYS439、DYS635、DYS393、Y-GATA-H4、DYS437、DYS438、DYS448各位点遗传多样性(GD值)分布在0.5300-0.9412之间;17个Y-STR位点共观察到单倍型95种,其单倍型多样性GD值为0.9998;97例哈萨克族Y-STR基因座各位点遗传多样性(GD值)分布在0.2769-0.8643之间;17个Y-STR位点共观察到单倍型95种,其单倍型多样性GD值为0.9996;结论:新疆和田地区维吾尔族17个Y-STR位点具有丰富的遗传多样性,选用ABI公司AmpFISTR-YfilerTM试剂盒,可为父权鉴定和父系进化研究提供有价值的遗传学资料;阿勒泰地区哈萨克族17个Y-STR基因座个别位点遗传多样性较差(GD值0.5),可以进一步扩大样本量来进一步验证YfilerTM试剂盒的17个Y-STR位点在该民族中的遗传多样性。
[Abstract]:Objective: to study the genetic polymorphism of 17 Y-STR loci in male Y chromosome of Uygur nationality and Kazak nationality in Hotan region, Xinjiang, and to reveal the genetic polymorphism and obtain the gene frequency of each locus.To understand the difference of individual recognition rate of each locus between the two nationalities, and to discuss the application of Yfiler kit in forensic medicine.At the same time, it provides basic data for the calculation of haplotype frequency and cumulative individual identification rate of 17 Y-STR of Uygur and Kazak ethnic groups in Hotan and Altay areas by Xinjiang public security organs.Methods: the template DNA was extracted by Chelex method from 96 Uygur and 97 Kazakh unrelated individual blood samples, and the five-color fluorescence multiplex amplification system of AmpFISTR-YfilerTM kit of ABI Company was used. 17 Y-STR loci could be amplified simultaneously in one reaction tube.ABI 3130XL genetic analyzer was used to detect and analyze the amplified products, and Data Collection 3.0 software was used to collect data from GeneMapper ID v3.3. The results of Y-STR typing were obtained.The GD value of haplotype diversity was 0.99989.97 Y-STR loci of Kazakh nationality were distributed in the range of 0.2769-0.8643, and 95 haplotypes were observed at 17 Y-STR loci.The GD value of haplotype diversity was 0.9996.Conclusion: 17 Y-STR loci of Uygur nationality in Hetian area of Xinjiang have abundant genetic diversity. The selection of AmpFISTR-YfilerTM kit of ABI company can provide valuable genetic data for paternity identification and paternal evolution.The genetic diversity of individual loci of 17 Y-STR loci of Kazak nationality in Altay region is poor and GD value is 0.5%, which can further expand the sample size to further verify the genetic diversity of 17 Y-STR loci of YfilerTM kit in this nationality.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R394

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