羊纤维环部分缺损动物模型的构建

发布时间：2018-04-17 02:16

本文选题：模型 + 动物　；参考：《天津医药》2016年05期

【摘要】：目的建立可用于椎间盘缺损修复研究的纤维环部分缺损动物模型。方法使用image J 1.46r软件测量5根羊脊柱X线片T_12/L1~L6/S1椎间隙高度,轴向剖开T_12/L1~L6/S1椎间盘,测量拟作缺损处纤维环厚度,使用11号尖刀在3根离体羊脊柱T_12/L1~L5/6椎间盘左前方制作上底3 mm、下底5 mm、高5 mm、厚3 mm,下底朝向髓核的梯形缺损,上述羊脊柱均取自1岁龄绵羊。采用微创外侧手术入路在2只1岁龄绵羊T_12/L1~L5/6椎间盘左前方制作相同规格的梯形缺损。通过轴向剖开椎间盘测量梯形缺损的边长、对切取的纤维环和少量髓核称质量,以此评估所制作的椎间盘梯形缺损。结果绵羊腰椎间隙高度为(4.45±0.28)mm,拟作缺损处纤维环厚度为(4.08±0.50)mm,分别与拟作梯形缺损的厚度(3 mm)和高度(5 mm)差异有统计学意义(P0.05);离体羊腰椎间盘梯形缺损的上底(3.03±0.09)mm、下底(5.03±0.09)mm、高度(4.97±0.10)mm和厚度(3.02±0.06)mm分别与梯形缺损预定值(上底3mm、下底5 mm、高度5 mm和厚度3 mm)之间的差异无统计学意义(P0.05);从离体和在体的羊腰椎间盘梯形缺损处取出的纤维环及髓核质量分别为(0.162±0.011)g和(0.166±0.014)g,两者差异无统计学意义(P0.05)。结论通过微创外侧手术入路,使用实验中制作梯形缺损的方法可以简单、安全、可靠地制作出符合要求、能用于椎间盘缺损修复研究的羊纤维环部分缺损的动物模型。
[Abstract]:Objective to establish an animal model of partial defect of fibrous ring which can be used for repair of disc defect.Methods image J 1.46r software was used to measure the height of T_12/L1~L6/S1 intervertebral space in 5 sheep spine X-ray films. The T_12/L1~L6/S1 intervertebral disc was dissected in axial direction, and the thickness of the fibrous ring in the defect was measured.A trapezoidal defect of the upper bottom 3 mm, the lower bottom 5 mm, the height 5 mm, the thickness 3 mm and the lower bottom towards the nucleus pulposus was made with the 11 sharp knife in the left front of the T_12/L1~L5/6 intervertebral disc of three isolated sheep, all of which were taken from 1 year old sheep.A trapezoid defect of the same size was made at the left front of T_12/L1~L5/6 disc in 2 1 year old sheep by minimally invasive lateral approach.The side length of trapezoid defect was measured by axial dissecting, and the obtained fibrous ring and a small amount of nucleus pulposus were weighed to evaluate the trapezoid defect of intervertebral disc.Results the height of lumbar intervertebral space in sheep was 4.45 卤0.28 mm, and the thickness of fibrous ring in the defect was 4.08 卤0.50 mm, which was significantly different from that of trapezoidal defect (3 mm) and height of 5 mm (P < 0.05), and the upper bottom of trapezoid defect of sheep lumbar intervertebral disc was 3.03 卤0.09 mm, and the lower floor was 5.03 卤0.09 mm.There was no significant difference in height 4.97 卤0.10)mm and thickness 3.02 卤0.06)mm between trapezoid defect and trapezoidal defect (upper bottom 3 mm, lower bottom 5 mm, height 5 mm and thickness 3 mm).The mass of nucleus pulposus was 0.162 卤0.011 g and 0.166 卤0.014 g, respectively. There was no significant difference between the two groups (P 0.05).Conclusion the method of trapezoid defect made by minimally invasive lateral surgical approach is simple, safe and reliable to make the animal model of partial defect of sheep fiber ring which can be used to repair the defect of intervertebral disc.
【作者单位】：天津医科大学研究生院;天津市天津医院;
【基金】：国家自然科学基金资助项目(81272046,31300798,31470937,31500781) 天津市卫生局攻关课题(14KG121,15KG125) 天津市科学技术委员会课题(15JCYBJC25300,11JCYBJC10200) 中国博士后科学基金项目(2012T50235)
【分类号】：R-332;R681.5

【相似文献】