六种固定液对小鼠角膜固定效果的比较

发布时间：2018-04-17 03:39

本文选题：小鼠 + 角膜　；参考：《中国比较医学杂志》2017年06期

【摘要】：目的观察6种不同固定液固定下,小鼠角膜的组织形态学特征并筛选出适合角膜固定的固定液。方法取60只NIH小鼠眼球,随机分为6组,每组10只,分别固定于6种固定液中,即95%乙醇、10%中性甲醛、Bouin’s液、甲醛-乙酸固定液和Davidson’s液。固定完成后,标本经80%、95%、100%梯度酒精脱水,TO型生物制片透明剂透明,石蜡浸泡,最后石蜡包埋。石蜡切片厚度均为4μm,分别做HE染色,显微镜下观察形态学特征并对角膜各区域厚度进行测量分析。结果不同固定液固定的小鼠角膜组织形态及厚度各具特征,而甲醛-乙酸液固定后的角膜组织细胞形态改变小。结论甲醛-乙酸液固定后组织细胞形态改变小,是比较合适的小鼠角膜固定液。
[Abstract]:Objective to observe the histomorphologic characteristics of the cornea of mice fixed with 6 different fixative fluids and to screen the suitable fixative solution for corneal fixation.Methods 60 NIH mice were randomly divided into 6 groups (n = 10 in each group), which were fixed in 6 kinds of fixative solution, namely 95% ethanol 10% neutral formaldehyde Bouins solution, formaldehyde-acetic acid fixative solution and Davidson's solution.After fixation, the specimen was treated with 100% gradient alcohol dehydration and 100% gradient alcohol dehydration. The transparent agent was transparent, soaked in paraffin, and finally embedded in paraffin.The thickness of paraffin sections was 4 渭 m. He staining was performed respectively. Morphological characteristics were observed under microscope and the thickness of each area of cornea was measured and analyzed.Results the corneal histomorphology and thickness of mice fixed with different fixations were different, but the corneal histocytes changed slightly after formaldehyde-acetic acid fixation.Conclusion formaldehyde-acetic acid solution is suitable for the corneal fixation of mice.
【作者单位】：广东省职业病防治院广东省职业病防治重点实验室;南方医科大学公共卫生与热带医学学院毒理学系;中山大学中山眼科中心眼科学国家重点实验室;
【基金】：广东省医学科学技术研究基金(编号:A2016122) 广东省职业病防治重点实验室(2012A061400007)
【分类号】：R361.2

【相似文献】