老年期痴呆同病异证3个典型证候Tau蛋白磷酸化位点的特异性研究

发布时间：2018-04-17 11:27

本文选题：老年期痴呆 + 证候　；参考：《北京中医药大学学报》2017年06期

【摘要】：目的观察3个老年期痴呆病证结合典型证候模型的大鼠Tau蛋白磷酸化位点的情况,分析总结其磷酸化的特异性。方法间隔1周结扎并剪断左、右颈总动脉,之后腹腔注射D-半乳糖,建立老年期痴呆病大鼠模型,最后在疾病模型的基础上分别进行房劳水劳刺激、投喂高脂饲乳和低温环境刺激,建立3种病证结合(肾虚精亏证、痰浊阻窍证和寒凝血瘀证)的老年期痴呆大鼠模型,通过测定血脂、血流变、血清睾酮水平检查验证各组模型是否成功。随后通过蛋白免疫印迹的方法观察Tau各磷酸化位点在各证候模型中的不同。结果在Tau蛋白脯氨酸富集区,与病理组相比,痰浊阻窍证组主要在205和231位磷酸化水平显著增强,肾虚精亏证组主要在181、205、231位磷酸化水平显著增强,寒凝血瘀证组磷酸化水平增强主要发生在205、231、262位;在C末端区域,与病理组相比,痰浊阻窍证组在404位磷酸化水平显著升高,肾虚精亏证组在396和404位磷酸化水平均显著升高,寒凝血瘀证组两者的升高幅度最高。结论老年期痴呆各典型证候在Tau蛋白磷酸化位点上有特异性,揭示了同病异证的科学内涵。
[Abstract]:Objective to observe the phosphorylation sites of Tau protein in three rat models of senile dementia combined with typical syndromes, and to analyze the specificity of phosphorylation.Methods Rat models of senile dementia were established by ligating and cutting off left and right common carotid arteries at intervals of 1 week, and then intraperitoneally injected with D-galactose.The rat model of senile dementia was established by feeding high fat feeding milk and hypothermia environment stimulation. Three kinds of disease syndromes (deficiency of kidney, stagnation of phlegm and obstruction of resuscitation and cold coagulation and blood stasis) were established. Blood lipids and hemorheology were measured.Serum testosterone level was tested to verify the success of each group.Then the phosphorylation sites of Tau in different syndromes were observed by Western blotting.Results in the Tau protein proline rich area, compared with the pathological group, the phosphorylation level in the phlegm turbid obstruction syndrome group was significantly increased at the 205th and 231th position, and the phosphorylation level was significantly increased in the kidney deficiency essence deficiency syndrome group at 181205231 position.In the C terminal region, the phosphorylation level in the phlegm turbid stagnation syndrome group was significantly higher than that in the pathological group, and the phosphorylation level in the kidney deficiency and essence deficiency group was significantly higher than that in the pathological group.The increase range of cold coagulation and blood stasis syndrome group was the highest.Conclusion the typical syndromes of senile dementia have specificity on Tau protein phosphorylation site, which reveals the scientific connotation of the same disease and different syndromes.
【作者单位】：山西中医学院;
【基金】：国家自然科学基金项目(No.81173165)~~
【分类号】：R277.7;R-332

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