长沙市沙门菌表型特征及PFGE分子分型

发布时间：2018-04-18 03:36

本文选题：沙门菌 + 血清学　；参考：《实用预防医学》2016年01期

【摘要】：目的研究长沙市沙门菌的血清型分布、耐药特点及分子流行病学特征。方法对分离自长沙地区食源性疾病粪便标本及市售食品中的70株沙门菌,用玻片凝集试验确定血清型,微量肉汤稀释法检测14种抗生素的敏感性,脉冲场凝胶电泳(PFGE)进行分子分型。结果鼠伤寒沙门菌为优势血清型,占51.43%,其次是肠炎沙门菌,占7.15%。在抗生素敏感性测试中,对氨苄青霉素、磺胺嘧啶和氯霉素的耐药率较高,分别为68.57%、61.43%和57.14%。62.86%(44/70)的沙门菌呈多重耐药,且鼠伤寒沙门菌的多重耐药率比其他血清型高,差异有统计学意义(χ2=7.068,P0.01)。PFGE分型结果显示69株沙门菌按照100%的相似度可分为57个PFGE型,36株鼠伤寒沙门菌可分为4个聚类,相似度在58.1%~100%之间。结论本市的沙门菌以鼠伤寒和肠炎沙门菌为主,PFGE分型结果显示可能存在沙门菌的流行株,且菌株耐药形势严峻。
[Abstract]:Objective to study the distribution of serotype, drug resistance and molecular epidemiology of Salmonella in Changsha.Methods 70 strains of salmonella were isolated from fecal specimens of foodborne diseases and marketed food in Changsha area. The serum type was determined by slide agglutination test. The sensitivity of 14 antibiotics was detected by broth dilution method.The molecular typing was carried out by pulsed field gel electrophoresis (PFGE).Results Salmonella typhimurium was the dominant serotype, accounting for 51.43, followed by Salmonella enteritidis, accounting for 7.15i.In the antibiotic sensitivity test, the resistant rates of ampicillin, sulfadiazine and chloramphenicol were 68.57% and 57.14.62.86%, respectively. The multidrug resistance of Salmonella typhimurium was higher than that of other serotypes, and the resistance rate of ampicillin, sulfadiazine and chloramphenicol was 68.57% and 57.14.62.86% respectively, and the resistance rate of salmonella typhimurium was higher than that of other serotypes.The difference was statistically significant (蠂 ~ 2 / 7.068 / P0.01N. PFGE typing results showed that 69 strains of Salmonella spp.) could be divided into 57 PFGE type and 36 strains of Salmonella typhimurium according to the similarity of 100%, and the similarity was between 58.1% and 100%.Conclusion the results of PFGE genotyping of Salmonella typhimurium and Salmonella enteritidis in this city indicate that there may be epidemic strains of Salmonella, and the situation of drug resistance of the strains is severe.
【作者单位】：长沙市疾病预防控制中心;湖南省疾病预防控制中心;
【基金】：湖南省卫生厅科研项目(B2014-130)
【分类号】：R378.22

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