复方麦芽丸对多囊卵巢综合征模型大鼠LH、FSH表达的影响

发布时间：2018-04-19 00:00

本文选题：多囊卵巢综合征 + 复方麦芽丸　；参考：《天然产物研究与开发》2017年02期

【摘要】：本文旨在研究复方麦芽丸(Compound Malt Pill,CMP)对多囊卵巢综合征(polycystic ovarian syndrome,PCOS)大鼠黄体生成素(LH)、促卵泡激素(FSH)表达的影响。通过运用9日龄SD大鼠颈背部皮下注射丙酸睾丸酮建立PCOS模型,造模成功的大鼠随机分为模型组,达英-35组和CMP低、中、高剂量组(n=9),另设正常组(n=10)。各治疗组以相应药物灌胃,正常组及模型组灌以等量的蒸馏水。运用免疫组化、PCR测定卵巢组织LH、FSH的蛋白及mRNA表达。结果显示,模型组中LH的蛋白及mRNA表达较正常组显著升高(P0.01),CMP各剂量组LH蛋白及mRNA表达较模型组显著降低(P0.01),达英-35组LH蛋白表达较模型组显著降低(P0.01),mRNA表达较模型组无显著差异(P0.05);模型组中FSH的蛋白及mRNA表达较正常组显著降低(P0.01),各治疗组FSH蛋白及mRNA表达较模型组显著升高(P0.01),研究结果表明,PCOS模型大鼠LH表达明显升高,FSH表达明显降低;复方麦芽丸对PCOS有治疗作用,可能是通过调节LH、FSH表达水平实现的。
[Abstract]:The purpose of this study was to investigate the effect of compound malt pill compound Malt (CMP) on the expression of luteinizing hormone (LHH) and follicle stimulating hormone (FSH) in polycystic ovarian syndrome (PCOS) rats.The PCOS model was established by subcutaneous injection of testosterone propionate into the neck and back of 9-day-old SD rats. The successful rats were randomly divided into three groups: model group, Daying -35 group, low, middle and high CMP group, and normal group.Each treatment group was gavaged with corresponding drugs, while the normal group and model group were treated with distilled water of the same amount.The protein and mRNA expression of LHH FSH were detected by immunohistochemistry.The results show thatThe expression of LH protein and mRNA in the model group was significantly higher than that in the normal group. The expression of LH protein and mRNA in each dose group of P0.01C group was significantly lower than that in the model group, while the expression of LH protein in the daiying -35 group was significantly lower than that in the model group. There was no significant difference in the expression of LH protein and mRNA between the model group and the model group.The expression of FSH protein and mRNA in the model group was significantly lower than that in the normal group, and the expression of FSH protein and mRNA in each treatment group was significantly higher than that in the model group. The results showed that the expression of LH in the model group was significantly higher than that in the control group.Compound malt pill has therapeutic effect on PCOS, which may be achieved by regulating the expression level of PCOS.
【作者单位】：重庆医科大学中医药学院中医药研究室;重庆市第十三人民医院;
【基金】：重庆市卫生局重点课题(ZY-20131003)
【分类号】：R285.5;R-332

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