3种流感病毒检测方法的比较

发布时间：2018-04-21 04:02

  本文选题：流感病毒 + 实时荧光PCR法　； 参考：《医学动物防制》2016年05期

【摘要】：目的对病毒分离培养法、实时荧光PCR法和鸡胚培养法3种检测流感病毒的方法和结果进行比较研究,筛选快速准确的检测方法,为流感监测与防控工作提供及时可靠的病原学依据。方法同时采用病毒分离培养法、实时荧光PCR法和鸡胚培养法对2014年10月~2015年3月牡丹江市流感监测哨点医院采集的流感样病例咽拭子样本进行检测,比较检测结果。结果实时荧光PCR法的流感病毒核酸检测阳性率为15.52%,其中169份为季节性H3N2流感病毒,73份为B型Yamagata株;病毒分离培养法的阳性率为6.29%,分离出63株季节性H3N2流感病毒,35株B型Yamagata株;鸡胚分离培养法检出率为0.64%。3种方法中,实时荧光PCR法的阳性率最高,病毒分离培养法次之,鸡胚分离培养法阳性率最低。结论实时荧光PCR法比病毒分离培养法和鸡胚培养法灵敏快捷、特异性强、敏感度高,适用于流感病毒的快速检测。
[Abstract]:Objective to compare the three methods and results of virus isolation and culture, real-time fluorescence PCR and chicken embryo culture, and to screen rapid and accurate methods for detection of influenza virus. To provide timely and reliable etiological basis for influenza surveillance and prevention and control. Methods simultaneously, virus isolation culture method, real-time fluorescence PCR method and chicken embryo culture method were used to detect the throat swabs of influenza cases collected from Mudanjiang influenza surveillance sentinel hospital from October 2014 to March 2015, and the results were compared. Results the positive rate of influenza virus nucleic acid detected by real-time fluorescent PCR method was 15.52, of which 169 were seasonal H3N2 influenza virus and 73 were type B Yamagata strain, and the positive rate of virus isolation culture method was 6.29 and 63 seasonal H3N2 influenza virus type B Yamagata strains were isolated. The positive rate of chicken embryo isolation culture method was 0.64.3, the positive rate of real-time fluorescence PCR method was the highest, virus isolation culture method was the second, and chicken embryo isolation culture method had the lowest positive rate. Conclusion the method of real-time fluorescence PCR is more sensitive, specific and sensitive than virus isolation culture and chicken embryo culture. It is suitable for rapid detection of influenza virus.
【作者单位】： 牡丹江市疾病预防控制中心;
【分类号】：R373.13;R446.5
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本文编号：1780825