炎性制剂对肝组织作用的动物实验研究

发布时间：2018-04-23 00:02

本文选题：松节油 + 豚鼠　；参考：《大理学院》2012年硕士论文

【摘要】：第一部分松节油肝内注射的剂量及实验动物的初步探讨材料与方法豚鼠6只，质量在350~420g之间，雌性，由大理学院实验动物中心提供。随机分成三组：A组为手术组，开腹手术，切除部分肝脏；另一只行肝门部阻滞；B组为开腹肝脏穿刺注射松节油；C组为超声下经皮穿刺注射松节油。结果 1A组一只豚鼠在术后关腹腔时，死亡。另一只在术中解除肝阻滞约5分钟后死亡。 2B组注射松节油0.3ml的豚鼠在术后24小时后死亡。第二只注射0.1ml的豚鼠麻醉后没有苏醒，死亡。 3C组在固定但没有麻醉的情况下，一只豚鼠在经皮肝穿刺时瞬间剧烈挣扎，致使穿刺针在胸腹腔内搅动，随即停止呼吸与心跳。另一只麻醉后，超声监测困难，穿刺出血多，只好放弃。结论 1将松节油注射于肝脏制作局部炎症时，豚鼠对松节油过于敏感，死亡率太高。 2豚鼠不适合常规下作经皮肝穿刺的实验动物。 3豚鼠创伤耐受性差，不利于术后的康复及实验观察，进一步改进实验！第二部分松节油、葡萄球菌混悬液肝内注射对兔肝组织作用的实验研究材料与方法本地成年健康家兔12只，质量在1.90~2.20kg之间，雌性，，由大理学院实验动物中心提供。随机分成四组：A组为对照组：无水酒精组；B组为松节油组；C组为葡萄球菌组；D组松节油、葡萄球菌混液系列组。结果 1A组为对照组：无菌生理盐水组，三只家兔中肝脏注射部位都无特殊变化。 2B组为松节油组，三只家兔中，有两只肝脏注射部位形成脓肿，一只出现局部炎症； 3C组为金黄色葡萄球菌组，有两只肝脏注射部位形成炎症，一只出现局部脓肿； 4D组为松节油-金黄色葡萄球菌悬液组，三只家兔中两只肝脏注射部位全部形成脓肿； 5十二只家兔，全部存活，肝脏出现脓肿或者炎症的，抗生素药物能控制。结论 1家兔作为经皮肝穿刺的实验动物、肝脏瘤内注射炎性制剂是安全的。 2松节油与葡萄球菌悬液序贯性瘤内注射可以使局部肝组织坏死、化脓；是理想的炎性制剂。 3肝脏瘤内内注射炎性制剂治疗肿瘤可能成为一种治疗模式！
[Abstract]:Part I preliminary study on intrahepatic injection of turpentine and experimental animals Materials and methods Six guinea pigs, weighing between 350 and 420 g, were provided by the Experimental Animal Center of Dali University. The rats were randomly divided into three groups: operation group (n = 3). Result One guinea pig in group 1A died after operation. The other died about 5 minutes after the liver block was lifted. Guinea pigs injected with turpentine 0.3ml died 24 hours after operation. The second guinea pig injected with 0.1ml did not wake up and died after anesthesia. In the 3C group, a guinea pig struggled violently during the percutaneous liver puncture, which caused the needle to stir in the chest and abdominal cavity, and then stopped breathing and heartbeat. Another anesthetized, ultrasound monitoring difficulties, puncture bleeding, had to give up. Conclusion 1 when the turpentine was injected into the liver to make local inflammation, the guinea pig was too sensitive to turpentine and the mortality was too high. 2 Guinea pigs are not suitable for routine percutaneous liver puncture. (3) the poor tolerance to trauma in guinea pigs is not conducive to postoperative rehabilitation and experimental observation, and further improvement of the experiment! The second part: the effect of intrahepatic injection of turpentine and staphylococcal suspension on rabbit liver tissue Empirical study Materials and methods Twelve local adult healthy rabbits, with mass between 1.90~2.20kg and female, were provided by the Experimental Animal Center of Dali University. The rats were randomly divided into four groups: control group: anhydrous alcohol group B group: turpentine group C group Staphylococcus group D group turpentine group and staphylococcal mixture group. Result Group 1A: control group: no special changes were found in the injection site of liver in three rabbits in aseptic saline group. Group 2B was turpentine group, two of the three rabbits had abscess at the injection site of liver and one had local inflammation. 3C group was staphylococcus aureus group, two livers were injected with inflammation and one had local abscess. 4Group D was turpentine-Staphylococcus aureus suspension group, and two of the three rabbits had abscess at the injection site of liver. Five-twelve rabbits, all alive, liver abscess or inflammation, antibiotics can be controlled. Conclusion 1 as the experimental animal of percutaneous liver puncture, it is safe to inject inflammatory agent into the liver. 2Sequential intratumoral injection of turpentine and staphylococcal suspension can make local liver tissue necrotic and suppurate, and is an ideal inflammatory preparation. 3Intrahepatic injection of inflammatory agents in the treatment of liver tumors may become a therapeutic model!
【学位授予单位】：大理学院
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R657.3;R-332

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