5种饮水对发育期大鼠骨代谢、骨微结构及骨强度影响的比较研究

发布时间：2018-04-23 11:02

本文选题：饮水 + 骨　；参考：《第三军医大学学报》2017年11期

【摘要】：目的比较天然矿物质水平不同的5种饮水对发育期大鼠骨骼生长的影响。方法 50只3周龄断乳SD大鼠适应性喂养1周后,按随机数字表法分为5组,分别饮用自来水(tap water,TP)、天然水(natural water,NW)、纯净水(purified water,PW)、天然矿泉水1(natural mineral water 1,NMW1)、天然矿泉水2(natural mineral water 2,NMW2),自由饮水及进食,喂养13周。每周测定大鼠体质量。实验结束后,取腹主动脉血分离血清,酶联免疫吸附法(ELISA)测定骨代谢生化指标;取左侧股骨,进行Micro-CT扫描及三维重建,观察松质骨骨微结构及检测相关参数,并采用三点弯曲法进行生物力学测定。指标各组间比较采用单因素方差分析,相关性采用Spearman相关性分析。结果与PW组相比,TP和NMW1、NMW2组的骨转换指标骨钙素(osteocalcin,OC)显著升高(P0.05)。与NW和PW组比较,NMW1和NMW2组的骨小梁强度增加,表现为骨表面积密度(bone surface/volume ratio,BS/BV)显著降低(P0.05),其余各指标组间差异无统计学意义。OC与水中溶解性总固体(total dissolved solids,TDS)、钙(Ca~(2+))、镁(Mg~(2+))、钾(K~+)呈正相关(r0.5,P0.05);BS/BV与TDS、Ca~(2+)、Mg~(2+)、K~+呈负相关(r-0.5,P0.05)。结论发育期大鼠长期饮用自来水和天然水后骨生长的差别不明显;饮用纯净水后骨生长水平较低,而饮用富含矿物质的天然矿泉水后对骨骼生长较有利。骨钙素和骨小梁强度的变化对水质较为敏感。
[Abstract]:Objective to compare the effects of 5 kinds of drinking water with different levels of natural minerals on skeletal growth in developing rats. Methods Fifty 3-week-old weaned SD rats were randomly divided into 5 groups after one week of adaptive feeding. Tap water, natural water, purified water, 1(natural mineral water, 2(natural mineral water 2NMW2, drinking water, drinking water, drinking water, drinking water, drinking water, drinking water, drinking water, drinking water, drinking water, drinking water, drinking water, drinking water, drinking water, drinking water, drinking water, drinking water, drinking water, drinking water, drinking water, drinking water, drinking water, drinking water, drinking water, drinking water, drinking water, drinking water, drinking water, drinking water and drinking water. The body mass of rats was measured weekly. At the end of the experiment, serum was isolated from abdominal aorta, biochemical indexes of bone metabolism were determined by enzyme-linked immunosorbent assay (Elisa), left femur was taken for Micro-CT scan and three-dimensional reconstruction, bone microstructure of cancellous bone was observed and relevant parameters were detected. The biomechanics was determined by three-point bending method. Single factor analysis of variance and Spearman correlation were used to compare the indexes among groups. Results compared with PW group, the osteocalcin OCs of TP and NMW1 + NMW2 groups were significantly higher than that of PW group (P 0.05). Compared with NW and PW groups, the bone trabecular strength of NMW1 and NMW2 groups increased, The results showed that the bone surface area density (surface/volume / BV) decreased significantly (P 0.05), but there was no significant difference between the other indexes. OC was negatively correlated with total dissolved solid dissolved (TDSs), Ca (2 +), mg (2) and K (K). Positive correlation was found between R0. 5 (P 0. 05) BS- / BV and TDS-0. 5P 0. 05 P 0. 05 (P < 0. 05), but no significant correlation was found between BSV and TDSV (0. 5P0. 05 P 0. 05 P 0. 05), and the correlation between BSV and TDSK was negative correlation (r 0. 5 P 0. 5P 0. 05 P 0. 05 P 0. 05 P 0. 05 P 0. 05 P 0. 05 P 0. 05). Conclusion there is no significant difference in bone growth between rats after drinking tap water and natural water for a long time, but the level of bone growth is lower after drinking pure water, but it is beneficial to bone growth after drinking mineral rich natural mineral water. The changes of osteocalcin and trabecular strength are sensitive to water quality.
【作者单位】：第三军医大学军事预防医学院军队环境卫生学教研室;
【基金】：国家自然科学基金重点项目(81230064)~~
【分类号】：R339.3

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