滋阴明目丸对视网膜光损伤大鼠模型Bcl-2及p53表达的影响

发布时间：2018-04-23 16:07

本文选题：Bcl- + p　；参考：《辽宁中医杂志》2017年05期

【摘要】：目的:通过观察滋阴明目丸对视网膜光损伤大鼠模型Bcl-2、p53表达的影响,探讨滋阴明目丸对视网膜光损伤大鼠模型的保护作用及治疗机制。方法:将60只Sprague-Dawley大鼠随机分为空白对照组、模型对照组、阳性对照组、滋阴明目丸低剂量组、滋阴明目丸中剂量组、滋阴明目丸高剂量组6组,每组10只。空白对照组不予处理,其余均建立视网膜光损伤大鼠模型,造模后第3天予以灌胃,连续15 d,其中滋阴明目丸低、中、高剂量组予以不同剂量滋阴明目丸灌胃,阳性对照组予以杞菊地黄丸灌胃。取大鼠眼球做免疫组化检测Bcl-2及p53的表达。结果:Bcl-2免疫组化结果:与空白对照组比较,模型对照组有统计学差异(P=0.0250.05);与模型对照组比较,阳性对照组(P=0.0030.01)、中剂量组(P=0.0040.01)及高剂量组(P=0.0010.01)均有显著统计学差异,低剂量组无明显差异(P0.05)。p53免疫组化结果:与空白对照组比较,模型对照组有显著统计学意义(P=0.0000.01);与模型对照组比较,低剂量组无明显差异(P0.05);阳性对照组(P=0.012)、中剂量组(P=0.002)及高剂量组(P=0.002)均有统计学差异(P0.05)。结论:视网膜光损伤模型可使大鼠视网膜组织抗凋亡蛋白Bcl-2表达降低,促凋亡蛋白p53表达增高,滋阴明目丸能有效增强Bcl-2的表达,降低p53的表达,从而对细胞凋亡起到双向调节作用,对视网膜光损伤模型具有一定的治疗作用。
[Abstract]:Objective: to investigate the protective effect and therapeutic mechanism of Ziyin Mingmu pill (ZYMW) on the expression of Bcl-2 p53 in rat model of retinal light injury. Methods: sixty Sprague-Dawley rats were randomly divided into blank control group, model control group, positive control group, low dose group, middle dose group and high dose group, with 10 rats in each group. No treatment was given in the blank control group. The other rats were given intragastric administration of Ziyin Mingmu pills for 15 days on the 3rd day after the establishment of the model. The middle and high dose groups were given different doses of Ziyin Mingmu pills. The positive control group was given Qiju Dihuang pills by stomach. The expression of Bcl-2 and p53 were detected by immunohistochemistry. Results: compared with the control group, the model control group had statistical difference (P < 0.01), the positive control group (P < 0.0030.01), the middle dose group (P < 0.0040.01) and the high dose group (P < 0.0010.01). There was no significant difference between the low dose group and the model control group (P 0.05N. P53 immunohistochemical results): compared with the blank control group, the model control group had significant statistical significance, and the model control group had significant statistical significance compared with the model control group. There was no significant difference between the low dose group and the high dose group (P 0.05), but there was a significant difference in the positive control group (P 0. 012), the middle dose group (P 0. 002) and the high dose group (P 0. 002). Conclusion: the model of retinal light injury can decrease the expression of anti-apoptotic protein Bcl-2 and increase the expression of apoptotic protein p53 in rat retina. Ziyin Mingmu pills can effectively enhance the expression of Bcl-2 and decrease the expression of p53. Thus, apoptosis plays a bidirectional regulatory role, and has a certain therapeutic effect on retinal light injury model.
【作者单位】：湖南中医药大学第一附属医院;湖南中医药大学;
【基金】：国家自然科学基金项目(81303007/H2713) 全国名老中医药专家李传课传承工作室(国中医药人教发2013(47)号)
【分类号】：R285.5;R-332

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