缺氧诱导因子-1α与基质金属蛋白酶-2在慢性压迫性颈脊髓损伤大鼠模型中的表达及意义

发布时间：2018-04-23 23:04

本文选题：脊髓损伤 + 慢性压迫　；参考：《中国脊柱脊髓杂志》2017年03期

【摘要】：目的 :探讨缺氧诱导因子-1α(hypoxia inducible factor-1α,HIF-1α)和基质金属蛋白酶-2(matrix metalloproteinases-2,MMP-2)在慢性压迫性颈脊髓损伤大鼠模型中的表达及意义。方法 :将80只成年SD大鼠随机分为假手术组和慢性压迫性颈脊髓损伤组(脊髓压迫组),每组40只。大鼠麻醉后充分显露C5和C6椎板,切除C5左侧半椎板,显露硬脊膜,脊髓压迫组在C6椎板和硬脊膜之间置入吸水后可膨胀聚氨酯薄板(1×3×1mm);假手术组只显露硬脊膜不置入压迫物。两组分别通过BBB(Basso Beattie Bresnahan)评分和体感诱发电位(somatosensory evoked potential,SEP)检测评估脊髓功能;于造模后7d、28d、42d和70d时处死大鼠取颈脊髓组织进行HIF-1α及MMP-2免疫组化染色,检测其在各时间点的表达量(IOD值),采用独立样本t检验比较两组各时间点的差异,分析HIF-1α、MMP-2与脊髓功能变化的相关性。结果:造模后7d时,两组BBB评分无显著性差异;SEP潜伏期显著性延长,波幅显著性降低;HIF-1α显著性降低,MMP-2显著性升高。28d时脊髓压迫组BBB评分显著性低于假手术组,SEP潜伏期与7d比较显著性延长(P0.05)、波幅显著性降低(P0.05),HIF-1α表达显著性增高(P0.05),而MMP-2表达显著性降低(P0.05)。42d时,脊髓压迫组BBB评分、SEP潜伏期和波幅与28d时比较无显著性差异,HIF-1α表达显著性增高(P0.05),而MMP-2表达显著性降低(P0.05);70d时脊髓压迫组神经功能较28d时显著性改善,BBB评分显著性增高(P0.05),SEP潜伏期显著性缩短(P0.05)、波幅显著性升高(P0.05);HIF-1α表达较前显著性降低,而MMP-2表达升高,但与假手术组比较仍有显著性差异(P0.05)。HIF-1α表达量与BBB评分呈显著性负相关(r=-0.458,P0.05),MMP-2表达量与BBB评分呈显著性正相关(r=0.903,P0.05)。结论:大鼠慢性压迫性脊髓损伤后具有一定程度的自我修复能力,HIF-1α、MMP-2表达变化与慢性脊髓压迫性损伤后神经功能改善相关。
[Abstract]:Objective: to investigate the expression and significance of hypoxia inducible factor-1 伪 (HIF-1 伪) and matrix metalloproteinases-2 (MMP-2) in chronic compressive cervical spinal cord injury (CSCI) rats. Methods: 80 adult SD rats were randomly divided into sham operation group and chronic compression cervical spinal cord injury group (40 rats in each group). After anesthesia, C5 and C6 laminae were fully exposed, the left half lamina of C5 was excised and the dura dural was exposed. In the spinal cord compression group, 1 脳 3 脳 1mm-1 mm expandable polyurethane thin plate was placed between the C6 lamina and the dura mater, and the sham operation group only exposed the dura mater not placed into the compression material. The spinal cord function was evaluated by BBB(Basso Beattie Bresnahan score and somatosensory evoked potentialsep, and the cervical spinal cord tissues were sacrificed for HIF-1 伪 and MMP-2 immunohistochemical staining on the 7th day, 28d, 42d and 70d, respectively. The expression of HIF-1 伪 MMP-2 was measured at different time points, and the correlation between HIF-1 伪 MMP-2 and spinal cord function was analyzed by t test. Results: there was no significant difference in BBB scores between the two groups at 7 days after modeling. The BBB score of spinal cord compression group was significantly lower than that of sham operation group (P 0.05), and the amplitude was significantly lower than that of sham operation group (P 0.05), while the expression of HIF-1 伪 in spinal cord compression group was significantly increased (P 0.05) and MMP-2 was significantly higher than that of sham operation group (P < 0.05), and the amplitude was significantly lower than that of sham operation group (P < 0.05), while the expression of HIF-1 伪 in spinal cord compression group was significantly higher than that in sham operation group (P < 0.05). The expression of P0. 05 and P0. 05 was significantly decreased on day 42, and the expression of P0. 05 was significantly decreased. There was no significant difference in latency and amplitude of BBB between spinal cord compression group and 28d group. The expression of HIF-1 伪 was significantly increased in spinal cord compression group. However, the expression of MMP-2 was significantly decreased at 70 days after spinal cord compression. The neurological function of spinal cord compression group was significantly improved compared with that at 28 days. The latent period of SEP was significantly shortened and the expression of HIF-1 伪 was significantly decreased. The expression of MMP-2 was increased, but there was still significant difference between the expression of P0.05U. HIF-1 伪 and BBB score. There was a significant negative correlation between the expression of MMP-2 and the BBB score. There was a significant positive correlation between the expression of MMP-2 and the BBB score. Conclusion: the expression of HIF-1 伪 and MMP-2 is related to the improvement of neural function after chronic compressive spinal cord injury in rats.
【作者单位】：中山大学附属第一医院脊柱外科;广东省骨科学重点实验室;
【分类号】：R651.2;R-332

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