骨髓间充质干细胞移植可增强衰老模型大鼠抗氧化能力和免疫活性

发布时间：2018-04-24 00:13

本文选题：间充质干细胞 + 衰老　；参考：《细胞与分子免疫学杂志》2017年02期

【摘要】：目的研究骨髓间充质干细胞(BMSC)移植对D-半乳糖致衰老大鼠抗氧化能力和免疫活性的影响。方法取10只健康雄性SD大鼠为青年对照组(2月龄)。另选取健康SD大鼠,每日皮下注射D-半乳糖(400 mg/kg),制备衰老模型。将衰老模型大鼠随机分为衰老模型组和BMSC治疗组(每组10只),治疗组给予尾静脉移植3×106个BMSC;同时对照组和模型组注射等量生理盐水。取各组大鼠血样本,检测血清中丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性。称胸腺质量计算各组大鼠胸腺指数;MTT法测定胸腺淋巴细胞转化指数;ELISA检测大鼠胸腺中白细胞介素2(IL-2)和IL-10含量。透射电镜观察各组大鼠胸腺组织的超微结构。结果 BMSC移植可提高大鼠血清SOD的活性,降低MDA含量。与衰老模型组相比,BMSC治疗组的胸腺指数、淋巴细胞转化指数升高。BMSC移植大鼠胸腺IL-2含量增加、IL-10含量降低。衰老模型组胸腺细胞排列稀疏,部分胞核固缩、凋亡;脂肪组织增多。而BMSC治疗能使胸腺细胞、网状上皮细胞的超微结构趋于正常,胞质内细胞器丰富完整。结论 BMSC移植可增强大鼠抗氧化能力并增强免疫活性,从而延缓免疫衰老。
[Abstract]:Objective to study the effects of bone marrow mesenchymal stem cell transplantation (BMSC) on anti-oxidation and immune activity of aging rats induced by D-galactose. Methods Ten healthy male Sprague-Dawley rats were selected as control group. In addition, healthy SD rats were injected subcutaneously with Dgalactose 400 mg / kg per day to establish aging model. The aging model rats were randomly divided into aging model group (n = 10) and BMSC treatment group (n = 10). The rats in the treatment group were given 3 脳 106 BMSCs of caudal vein and the control group and the model group were injected with the same amount of normal saline at the same time. Serum malondialdehyde (MDA) and superoxide dismutase (SOD) activity were measured. The thymus index of each group was measured by MTT assay. The thymus lymphocyte transformation index (TLI) and the content of interleukin 2 (IL 2) and IL-10 in the thymus of rats were determined by Elisa. The ultrastructure of thymus tissue in each group was observed by transmission electron microscope. Results BMSC transplantation could increase the activity of serum SOD and decrease the content of MDA. Compared with the aging model group, the thymus index and lymphocyte transformation index of BMSC treatment group increased. The IL-2 content of thymus increased and IL-10 content decreased in BMSC transplantation rats. In the aging model group, the thymocytes were arranged sparsely, some of the nuclei were pyknosis and apoptosis, and the adipose tissue was increased. BMSC treatment could make the ultrastructure of thymocytes and reticular epithelial cells normal, and the cytoplasmic organelles were abundant and complete. Conclusion BMSC transplantation can enhance the anti-oxidation ability and immune activity of rats and delay the immune aging.
【作者单位】：福建医科大学省立临床医学院福建省立医院血液科;
【基金】：福建省自然科学基金(2014J05084)
【分类号】：R339.38

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