游泳运动对D-半乳糖致衰老小鼠学习记忆和胆碱能神经系统功能的影响

发布时间：2018-04-25 04:23

本文选题：游泳运动 + D-半乳糖　；参考：《中国运动医学杂志》2016年01期

【摘要】：目的:探讨游泳运动对D-半乳糖所致衰老小鼠学习记忆和胆碱能系统功能的影响。方法:50只昆明种小鼠随机分成5组:对照组、模型组、15 min/天游泳组、30 min/天游泳组、45 min/天游泳组。除对照组外,所有小鼠连续8周皮下注射D-半乳糖复制衰老模型。运动组小鼠从第5周开始运动,连续4周。Morris水迷宫实验评价小鼠空间学习记忆能力。行为测试结束后,检测小鼠脑组织丙二醛(MDA)含量和超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)活性及皮层和海马组织中乙酰胆碱酯酶(ACh E)和胆碱乙酰化转移酶(Ch AT)的活性。结果:水迷宫实验结果显示:15 min/天和30 min/天游泳组逃避潜伏期显著短于模型组,目标象限搜索时间百分比、通过目标区域次数均显著多于模型组。酶活性检测结果显示:15min/天游泳组小鼠脑组织SOD显著高于模型组,GSH-Px活性高于模型组但无显著性,MDA含量显著低于模型组;30 min/天游泳组小鼠脑组织SOD和GSH-Px活性均显著高于模型组、MDA含量显著低于模型组;15 min/天和30 min/天游泳组皮层和海马组织中ACh E、Ch AT活性均显著高于模型组。结论:15 min/天游泳运动和30 min/天游泳运动均能提高衰老模型小鼠的空间学习记忆能力,其中以30 min/天游泳运动的效果最好,其机制可能与其提高脑组织抗氧化和清除自由基能力,增强中枢胆碱能神经系统功能有关。
[Abstract]:Aim: to investigate the effects of swimming on learning and memory and cholinergic system function in aging mice induced by D-galactose. Methods 50 Kunming mice were randomly divided into 5 groups: control group, model group, 30 min/ day swimming group, 30 min/ day swimming group, 45 min/ day swimming group. All the mice except the control group were subcutaneously injected with D-galactose for 8 weeks to make aging model. The exercise group began to exercise at the 5th week and the spatial learning and memory ability was evaluated by Morris water maze test for 4 weeks. After the behavior test, the contents of malondialdehyde (MDA), the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and the activities of acetylcholinesterase (ach) and choline acetyltransferase (Chat) in cortex and hippocampus were measured. Results: the results of water maze test showed that the escape latency of swimming group on 15 min/ and 30 min/ days was significantly shorter than that of model group, the percentage of target quadrant search time and the number of times of passing through target area were significantly higher than that of model group. The results of enzyme activity test showed that the activity of SOD in brain tissue of mice in 15 min / day swimming group was significantly higher than that in model group, but the content of SOD and GSH-Px in brain tissue of mice in 30 min/ day swimming group was significantly lower than that in model group 30 min/ day swimming group, but the activity of SOD and GSH-Px in brain tissue of model group was significantly higher than that of model group. The content of MDA in the model group was significantly lower than that in the model group on 15 min/ and 30 min/ days, and the activity of ACh Etchat in the cortex and hippocampus of the swimming group was significantly higher than that of the model group. Conclusion the spatial learning and memory ability of aging model mice can be improved by swimming on 15 min/ day and 30 min/ day swimming exercise, and 30 min/ day swimming exercise is the best. The mechanism may be related to the ability of antioxidant and free radical scavenging in brain tissue. Enhancing the function of central cholinergic nervous system.
【作者单位】：滨州医学院;
【分类号】：R338

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