两种慢性高眼压大鼠模型视网膜结构和眼压变化的评估

发布时间：2018-04-25 13:26

本文选题：慢性高眼压 + 青光眼　；参考：《眼科新进展》2017年07期

【摘要】：目的评估两种慢性高眼压大鼠模型的升眼压效果和视网膜结构的改变。方法分别通过前房内注射微珠(微珠组)和结扎3支巩膜上静脉(结扎组)的方法制作两种慢性高眼压大鼠模型(每组6只)。使用Tono Pen眼压计测量大鼠眼压,模型制作当天及其后每周测量大鼠眼压,大鼠右眼为实验眼,左眼为对照眼(假手术眼)。采用荧光金上丘逆标的方法标记视网膜神经节细胞并计数;使用免疫荧光标记的方法观察大鼠视网膜结构的改变。结果造模后1-8周微球组和结扎组大鼠眼压均升高;其中结扎组眼压为(27.20±1.83)mm Hg(1 kPa=7.5 mm Hg),其对照眼眼压为(19.80±1.35)mm Hg(P=0.001,n=6);微珠组眼压为(27.40±1.88)mm Hg,其对照眼眼压为(19.40±1.00)mm Hg(P=0.000,n=6)。造模后8周,巩膜上静脉结扎组视网膜神经节细胞丢失37.9%、39.6%和33.5%(均为P=0.000,n=6),前房内注射微珠组丢失37.3%、39.4%和32.3%(均为P=0.000,n=6),两组视网膜神经节细胞的丢失数量比较差异均无统计学意义(P=0.855、0.949、0.634,n=6)。高眼压模型8周后,相较于对照组,微珠组和结扎组视网膜神经节细胞核数量均有明显减少,组织厚度变薄,但两组间差异无统计学意义[对照组厚度(7.32±0.39)μm,结扎组厚度(4.97±0.33)μm,微珠组厚度(5.00±0.31)μm]。前房内注射微珠组在部分组织切片可发现微珠污染。结论巩膜上静脉结扎和前房内注射微珠均可以使大鼠眼压稳定增高。巩膜上静脉结扎具有实施方便和价格低的优势,并且没有微珠污染的缺点。
[Abstract]:Objective to evaluate the ocular hypertension and retinal structure changes in two chronic ocular hypertension rats. Methods two chronic ocular hypertension rats (6 rats in each group) were made by injecting microbeads (microbeads) in the anterior chamber and ligating 3 superior scleral veins (ligation group). The intraocular pressure of rats was measured by Tono Pen tonometer, and the model was made as a model. The rat eye pressure was measured every week, the right eye of the rat was taken as the experimental eye and the left eye was the control eye (sham operation eye). The retinal ganglion cells were marked with the method of fluorescent gold colliculus inversion, and the changes of the retinal structure were observed by immunofluorescent labeling. The intraocular pressure of the microsphere group and the ligation group of the 1-8 weeks after the formation of the fruit was both in the eye pressure of the rats. The intraocular pressure in the ligation group was (27.20 + 1.83) mm Hg (1 kPa=7.5 mm Hg), the intraocular pressure of the control eye was (19.80 + 1.35) mm Hg (P=0.001, n=6), and the intraocular pressure in the microsphere group was (27.40 + 1.88) mm Hg, and the control eye pressure was (19.40 + 1) mm. After 8 weeks, the retinal ganglion cells in the supra scleral vein ligature group lost 37.9%, 39.6% and 33.5%. P=0.000, n=6), 37.3%, 39.4% and 32.3% (all P=0.000, n=6) were lost in the anterior chamber injection microspheres. There was no significant difference in the number of retinal ganglion cells in two groups (P=0.855,0.949,0.634, n=6). After 8 weeks of high intraocular pressure model, the number of nuclei in the retinal ganglion cells in the microbead group and the ligation group were all clear. The thickness of the tissue was thinner, but there was no significant difference between the two groups [the thickness of the control group (7.32 + 0.39) mu m, the thickness of the ligation group (4.97 + 0.33) mu m, the thickness of the microbead group (5 + 0.31) m]. in the anterior chamber of the microspheres could be found in the microspheres in some tissue sections. Conclusion the supra scleral vein ligation and the anterior chamber injection of microspheres could make the rat eye The stability of the scleral vein ligation has the advantages of convenient implementation and low price, and there is no shortcoming of microbead pollution.

【作者单位】：河南中医药大学组胚学科;上海交通大学医学院附属瑞金医院北院眼科;
【分类号】：R-332;R775

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