千金骨康散对大鼠胫骨骨折模型的影响

发布时间：2018-04-25 18:02

本文选题：千金骨康散 + 胫骨骨折模型　；参考：《中国实验方剂学杂志》2017年03期

【摘要】：目的:研究大鼠胫骨骨折模型评价千金骨康散对骨折的治疗作用。方法:雄性SD大鼠40只,除正常组10只外,其余30只大鼠采用2%戊巴比妥钠腹腔麻醉,无菌操作于左下肢胫骨粗隆下约1 cm处,以骨剪横向将胫骨剪成3 mm宽,2 mm深的骨缺损,建立胫骨骨折模型,将造模大鼠随机分为3组,分别为模型组,千金骨康散低、高剂量组(0.35,0.7 g·kg-1),各组分别ig给药,给药体积为15 m L·kg-1,每天1次,连续给药21 d。实验末次给药后对大鼠左侧胫骨X射线成像,采血进行血液流变检测、取胫骨骨痂部位进行组织病理学检查。结果:与正常组比较,模型组大鼠有内外轻度骨痂的改变,有错位现象,大鼠血液黏度明显升高,大鼠骨折后骨痂部位组织病理学变化较为明显;与模型组比较,X成像显示千金骨康散低、高剂量能明显促进骨折愈合(P0.05,P0.01),高剂量能明显降低大鼠胫骨骨折模型血液黏度(P0.05);千金骨康散低、高剂量能明显改造大鼠骨折后骨痂部位组织病理学变化(P0.05,P0.01)。结论:千金骨康散对大鼠胫骨骨折具有一定的促愈合作用。
[Abstract]:Objective: to evaluate the therapeutic effect of Qianjin Gukang Powder on tibial fracture in rats. Methods: 40 male Sprague-Dawley rats, except 10 in normal group, were anesthetized with 2% pentobarbital sodium intraperitoneally and sterilized at about 1 cm below the protuberance of the tibia of the left lower extremity. The tibia was cut horizontally into a bone defect of 3 mm wide and 2 mm deep. The model rats were randomly divided into three groups: the model group was treated with Qianjin Gukang Powder (QJKS), the high dose group was treated with 0.35U 0.7 g kg-1g 路kg ~ (-1), the volume of each group was 15 mL / kg ~ (-1), once a day, and the drug was given continuously for 21 days. After the last administration of the drug, the left tibia of rats was examined by X-ray imaging, blood was collected for hemorheological examination, and the callus of tibia was taken for histopathological examination. Results: compared with the normal group, the rats in the model group had slight internal and external callus changes, dislocation, increased blood viscosity and histopathological changes of the callus after fracture. Compared with the model group, the X-ray imaging showed that Qianjin Gukang San was low, high dose could obviously promote fracture healing, high dose could obviously reduce the blood viscosity of tibial fracture model in rats (P 0.05), and Qianjin Gu Kang San was low. The histopathological changes of callus after fracture could be significantly modified by high dose (P 0.05) and P 0.01 (P < 0.05). Conclusion: Qianjin Gukang Powder can promote the healing of tibial fracture in rats.
【作者单位】：湖南省药物安全评价研究中心;株洲千金药业股份有限公司;北京协和医学院药用植物所;
【基金】：湖湘青年科技创新创业人才基金项目(2014)
【分类号】：R285.5;R-332

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