抑郁模型大鼠海马组织中内质网应激调控因子的表达及β-细辛醚的干预作用

发布时间：2018-04-28 03:20

本文选题：β-细辛醚 + 抑郁　；参考：《中国实验方剂学杂志》2017年08期

【摘要】：目的:研究抑郁模型大鼠海马组织中内质网应激调控因子的表达及β-细辛醚的干预作用,并探讨其相关的作用机制。方法:建立慢性轻度不可预见性应激刺激(CUMS)大鼠模型,将100只大鼠随机分为5组,分别为正常组,模型组,氟西汀组(10 mg·kg-1),β-细辛醚低剂量组(25 mg·kg-1)和高剂量组(50 mg·kg-1),每组20只,从造模第8天开始每天灌胃1次给药,连续21 d。于实验的第0天和第29天进行旷场实验;采用Nissl染色法观察各组大鼠海马组织神经元尼氏体的变化;实时荧光定量PCR(Real-time PCR)技术检测各组大鼠海马组织蛋白激酶R样内质网激酶(PERK),CCAAT/增强子结合蛋白同源蛋白(CHOP),钙网蛋白(CRT)mRNA表达情况;蛋白质免疫印迹(Western blot)技术检测各组大鼠海马组织PERK,CHOP,CRT蛋白表达。结果:经28 d慢性不可预见性温和刺激后,模型组大鼠与正常组大鼠比较,水平运动和垂直运动得分明显减少(P0.05);β-细辛醚低、高剂量组和氟西汀组大鼠与模型组比较,水平运动和垂直运动得分明显增加(P0.05)。尼氏染色结果显示,模型组大鼠海马组织神经元细胞浆着色变浅,尼氏小体颗粒明显减少;β-细辛醚低、高剂量组大鼠海马区神经元形态相对较好,尼氏小体颗粒较模型组增多。模型组与正常组大鼠比较,PERK,CHOP,CRT mRNA表达上调(P0.05),与模型组比较,β-细辛醚低、高剂量组和氟西汀组中PERK,CHOP,CRT mRNA表达明显下调(P0.05)。与正常组比较,模型组大鼠PERK,CHOP,CRT蛋白表达均明显升高(P0.05);与模型组大鼠比较,氟西汀组、β-细辛醚低、高剂量组PERK,CHOP,CRT蛋白表达明显减少(P0.05)。结论:β-细辛醚可能是通过调节海马内质网功能发挥抗抑郁作用。
[Abstract]:Aim: to investigate the expression of endoplasmic reticulum (ER) stress regulatory factors and the intervention of 尾 -asarone in hippocampus of depression rats and to explore its related mechanism. Methods: chronic mild unpredictable stress stimulation (CUMS) rat model was established. 100 rats were randomly divided into 5 groups: normal group, model group, fluoxetine group (10 mg 路kg ~ (-1), 尾 -asarone group (25 mg 路kg ~ (-1) and high dose group (50 mg 路kg ~ (-1). From the 8th day, the rats were given orally once a day for 21 days. The open field experiment was carried out on day 0 and day 29, and the changes of Nissl bodies in hippocampal neurons were observed by Nissl staining. The expression of protein kinase R-like endoplasmic reticulum kinase (PERK) / enhancer protein homologue protein (CHOPN) and calcium reticulum protein (CRT) mRNA in hippocampal tissues of rats in each group were detected by real-time fluorescence quantitative PCR(Real-time. Western blotting technique was used to detect the CRT expression in rat hippocampal tissue. Results: after 28 days of chronic unpredictable mild stimulation, the scores of horizontal and vertical movements in the model group were significantly lower than those in the normal group, and the scores of 尾 -asarone were lower than those in the model group, while those in the high dose group and fluoxetine group were significantly lower than those in the model group. The scores of horizontal and vertical movements increased significantly (P 0.05). The results of Nissl staining showed that the cytoplasm of hippocampal neurons in the model group was shallower, and the granules of Nissl corpuscles were significantly decreased, while 尾 -asarone was low, and the morphology of neurons in the hippocampal area of the high dose group was relatively good, and the number of the granules in the hippocampal area of the model group was higher than that in the model group. Compared with the normal group, the expression of PERKG CHOPOCRT mRNA was up-regulated in the model group, and the expression of 尾 -asarone was lower in the model group than in the model group. The expression of PERKTCHOPOP-CRT mRNA in the high dose group and fluoxetine group was significantly down-regulated as compared with the model group, and the expression of P0.05T was down-regulated in the high dose group and fluoxetine group. Compared with the normal group, the expression of PERKG CHOPCRT protein in the model group was significantly higher than that in the model group, and in fluoxetine group, 尾 -asarone was lower than that in the high dose group, and the expression of PERK- CHOPP-CRT protein in the high dose group was significantly lower than that in the model group. Conclusion: 尾-asarone may play an antidepressant role by regulating the endoplasmic reticulum function in the hippocampus.
【作者单位】：齐齐哈尔医学院;
【基金】：黑龙江省科技厅攻关项目(PC13S06)
【分类号】：R285.5;R-332

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