基于免疫吸附柱用磁性涂层纤维的制备及其吸附性能研究

发布时间：2018-04-29 07:28

本文选题：免疫吸附 + 活性碳纤维　；参考：《中南大学》2011年硕士论文

【摘要】：免疫吸附治疗技术已成为去除血液中致病物质的一种重要手段,免疫吸附剂是决定治疗效果的关键因素,有别于珠状或膜状免疫吸附剂,免疫吸附碳纤维是一种有重要应用前景的血液净化吸附剂。本文对碳纤维进行表面磁性处理和活化改性,制备出免疫吸附碳纤维并组装出吸附柱样件,对免疫吸附纤维及吸附柱样件的吸附性能进行了研究。制备了磁性涂层碳纤维,对其晶体结构、表面形貌、磁性能以及力学性能进行表征,XRD.SEM和EDS结果表明：磁性涂层碳纤维存在C和Fe304两种晶相,Fe304在磁性涂层碳纤维表面包覆情况较好,磁性涂层碳纤维含有C、Fe和O元素,所占的质量百分比分别是79.05%、18.54%和2.41%；VSM结果显示：磁性涂层碳纤维的比饱和磁化强度和矫顽力分别为3.273emu/g(?)口218.2350e;拉伸强度测试结果显示：磁性涂层碳纤维的单丝强度为4.45Gpa。对磁性涂层碳纤维采用戊二醛法和氨基硅烷(KH550)法进行化学活化偶联,IR结果表明：在磁性涂层碳纤维表面引入了羟基、羧基和氨基,使其能有效键连蛋白、抗体、DNA等各种配基。静态平行实验结果表明：经活化偶联改性的磁性涂层碳纤维对BSA的吸附容量远高于空白碳纤维和单一的磁性涂层碳纤维,其中KH550偶联改性法制备的磁性涂层碳纤维对BSA的吸附容量最大,达到93.73mg/g。免疫吸附纤维对BSA的吸附行为大致符合Langmuir吸附模型,理论饱和吸附量为104.49mg/g,饱和吸附时间为120min。动态吸附实验结果表明：免疫吸附纤维柱对BSA的最大吸附容量为30.73mg/g,相比静态吸附的吸附容量有明显降低。动物实验结果显示：血液中的胆固醇和LDL含量经免疫吸附纤维吸附后略有降低,呈阴性。
[Abstract]:Immunosorbent therapy has become an important means of removing pathogenic substances in blood. Immune adsorbent is the key factor to determine the therapeutic effect, which is different from bead or membrane immune adsorbent. Immunoadsorbed carbon fiber is an important adsorbent for blood purification. In this paper, the surface magnetic treatment and activation modification of carbon fiber were carried out to prepare the adsorbed carbon fiber and to assemble the adsorbed column sample. The adsorption properties of the immunosorbent fiber and the adsorption column sample were studied. Magnetic coated carbon fibers were prepared, and their crystal structure and surface morphology were investigated. The magnetic properties and mechanical properties were characterized by XRD. SEM and EDS. The results show that there are two kinds of crystalline phase (C and Fe304) in the magnetic coated carbon fiber. The magnetic coated carbon fiber contains CnFe and O elements. The specific saturation magnetization and coercivity of the magnetically coated carbon fibers were 3.273 emu / g / g respectively (79.05% 18.54% and 2.41% VSM). The tensile strength of magnetic coated carbon fiber was 4.45 Gpa. The results of chemical activation coupling of magnetic coated carbon fibers by glutaraldehyde and aminosilane KH 550 showed that hydroxyl groups, carboxyl groups and amino groups were introduced on the surface of magnetic coated carbon fibers to enable them to effectively bind proteins, antibody DNA and other ligands. The results of static parallel experiments show that the adsorption capacity of magnetic coated carbon fiber modified by activated coupling is much higher than that of blank carbon fiber and single magnetic coated carbon fiber. The magnetic coated carbon fiber prepared by KH550 coupling modification has the largest adsorption capacity for BSA, reaching 93.73 mg / g. The adsorption behavior of immunosorbent fiber for BSA was approximately in line with the Langmuir adsorption model. The theoretical saturated adsorption capacity was 104.49 mg / g and the saturation adsorption time was 120 min. The experimental results of dynamic adsorption show that the maximum adsorption capacity of BSA on the immunosorbent fiber column is 30.73 mg / g, which is obviously lower than that of static adsorption. The results of animal experiments showed that the contents of cholesterol and LDL in blood decreased slightly after adsorption with immunosorbent fibers and were negative.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R392.1

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