重组人促红细胞生成素对衰老大鼠脑组织不同部位BDNF表达的影响

发布时间：2018-04-29 09:29

本文选题：脑源性神经生长因子 + 重组人促红细胞生成素　；参考：《南方医科大学学报》2017年04期

【摘要】：目的探讨重组人促红细胞生成素(rh EPO)对衰老大鼠脑组织内不同部位脑源性神经营养因子(BDNF)表达的影响。方法40只2月龄雄性SD大鼠,随机分为4组:阴性对照组(N),D-半乳糖组(D),EPO干预组(E),阳性对照组(P),每组10只。应用免疫组化染色对比观察外源性rh EPO干预后D-半乳糖衰老大鼠脑组织不同部位BDNF表达的变化。结果不同组大鼠比较发现相同部位BDNF表达存在显著差异:D组大鼠海马CA1、CA3、DG及额叶皮质区BDNF阳性细胞计数较N组相同部位明显减少(P0.05),而应用rh EPO干预后的E组和P组大鼠海马CA1、CA3、DG及大脑皮质运动区BDNF阳性细胞计数较D组及N组相同部位显著增加(P0.05);但E组和P组比较无差异。同组大鼠不同部位BDNF阳性细胞比较发现:同组大鼠不同部位BDNF阳性细胞个数存在显著不同(P0.05),其中额叶皮质区阳性细胞数最多,其次是海马CA3区,海马DG区,海马CA1区。结论 rh EPO对增强大鼠神经细胞BDNF的表达具有普遍性,提示rh EPO可能能够通过增强内源性BDNF对神经系统衰老发挥保护作用。
[Abstract]:Objective to investigate the effect of recombinant human erythropoietin (rh EPO) on the expression of brain-derived neurotrophic factor (BDNF) in different parts of brain tissue of aging rats. Methods Forty 2-month-old male Sprague-Dawley rats were randomly divided into 4 groups: the negative control group (n = 10) and the control group (n = 10). Immunohistochemical staining was used to observe the changes of BDNF expression in different parts of brain tissue of aging rats induced by exogenous rh EPO. Results there were significant differences in the expression of BDNF in the same part of rats in different groups. The number of BDNF positive cells in hippocampal CA1 / CA3DG and frontal cortex of rats in group 1: d was significantly lower than that in group N, while the number of positive cells in group E and group P after the intervention of rh EPO was significantly lower than that in group N. The number of BDNF positive cells in hippocampal CA1 + CA3 + DG and cerebral cortex motor area in rats was significantly higher than that in group D and group N, but there was no difference between group E and group P. The comparison of BDNF positive cells in different parts of rats in the same group showed that there were significant differences in the number of BDNF positive cells in different parts of the same group, among which the number of positive cells in frontal cortex was the most, followed by CA3 in hippocampus, DG in hippocampus and CA1 in hippocampus. Conclusion rh EPO has a universal role in enhancing the expression of BDNF in rat nerve cells, suggesting that rh EPO may play a protective role in nervous system senescence by enhancing endogenous BDNF.
【作者单位】：西安交通大学医学院第二附属医院神经内科;
【基金】：国家自然科学基金(81170330)~~
【分类号】：R338

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