宁夏密点麻蜥对胃癌前病变模型大鼠P53及PUMA表达的影响

发布时间：2018-04-30 12:15

本文选题：宁夏密点麻蜥 + PLGC　；参考：《辽宁中医杂志》2017年07期

【摘要】：目的:研究宁夏密点麻蜥不同剂量对胃癌前病变(PLGC)模型大鼠胃黏膜细胞增殖、凋亡以及P53和PUMA水平的影响,探讨宁夏密点麻蜥对PLGC的干预机制。方法:60只SPF级雄性SD大鼠,随机分为空白对照组,模型组,宁夏密点麻蜥高、中、低剂量组,维霉素对照组,共6组,每组10只。除空白组外,余均以0.02 mmol/L的MNNG联合40%乙醇、饥饱失常等综合因素共24周制备PLGC大鼠模型。分别用宁夏密点麻蜥高、中、低剂量、维酶素连续治疗12周,观察PLGC模型大鼠胃黏膜病理组织学变化,应用免疫组化法分别观察给药前和停药后胃体组织P53和PUMA蛋白变化,并进行统计分析。结果:通过Image-Pro Plus 6.0图像分析系统对免疫组化图像进行光密度的测算,各模型组与空白对照组P53和PUMA水平比较,AOD值明显降低,P0.05;各治疗组与模型组P53和PUMA水平比较,AOD值明显降低,P0.05;宁夏密点麻蜥高、中、低剂量治疗组P53和PUMA阳性表达率低于维酶素治疗组,P0.05,其中以高剂量组AOD值最小。结论:宁夏密点麻蜥能抑制凋亡相关蛋白P53、PUMA的表达,促进细胞凋亡,逆转PLGC模型大鼠胃黏膜病理改变,阻断癌变发展。
[Abstract]:Aim: to study the effects of different doses of Leptanthus mildeus on gastric mucosal cell proliferation, apoptosis and p53 and PUMA levels in gastric precancerous lesion model rats, and to explore the mechanism of PLGC intervention. Methods Sixty male Sprague-Dawley rats of SPF grade were randomly divided into blank control group, model group, high, middle and low dose group of Ningxia Dendrolimus nigrum, and control group of Vitamin, with 10 rats in each group. In addition to the blank group, the PLGC rat model was established by the combination of 0.02 mmol/L MNNG and 40% ethanol, hunger and satiety disorder for 24 weeks. The histopathological changes of gastric mucosa in PLGC model rats were observed after 12 weeks of continuous treatment with high, medium and low doses of Vulinase, and the changes of p53 and PUMA protein in gastric body tissue before and after drug withdrawal were observed by immunohistochemical method. Statistical analysis was carried out. Results: the optical density of immunohistochemical images was measured by Image-Pro Plus 6.0 image analysis system. Compared with the blank control group, the levels of p53 and PUMA in each model group were significantly lower than those in the control group (P 0.05), the levels of p53 and PUMA in each treatment group were significantly lower than those in the model group (P 0.05). The positive expression rate of p53 and PUMA in low dose group was lower than that in vermin treatment group (P 0.05). The AOD value of high dose group was the lowest. Conclusion: Rupanthus mandshurica can inhibit the expression of apoptosis-related protein P53 and PUMA, promote apoptosis, reverse the pathological changes of gastric mucosa in PLGC rats, and block the development of carcinogenesis.
【作者单位】：宁夏医科大学;
【基金】：国家自然科学基金(81560769) 2015宁夏自然科学基金(NZ15089) 2014年度宁夏高等学校科学研究项目(NGY2014096)
【分类号】：R285.5;R-332

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