慢病毒介导稳定敲低Smurf1细胞株的构建及对细胞迁移的影响

发布时间：2018-04-30 16:40

  本文选题：Smurf + 慢病毒　； 参考：《中国比较医学杂志》2017年04期

【摘要】：目的构建慢病毒介导稳定敲低Smurf1的HeLa和A549细胞株并检测敲低Smurf1细胞迁移的影响。方法将包装好的Smurf1敲低慢病毒感染HeLa和A549细胞,7 d后进行嘌呤霉素抗性筛选阳性细胞,Western blot和qPCR检测敲低效果,并进行Transwell检测Smurf1敲低对细胞迁移的影响。结果利用干扰慢病毒系统成功构建稳定敲低Smurf1的HeLa和A549细胞株,稳定敲低Smurf1抑制细胞的迁移速率。结论敲低Smurf1抑制细胞迁移。
[Abstract]:Aim to construct HeLa and A549 cell lines with stable knockout Smurf1 mediated by lentivirus and to detect the migration of Smurf1 knockout cells. Methods HeLa and A549 cells were infected with Smurf1 knockdown virus for 7 days. Western blot and qPCR were used to screen the positive cells for purine mycin resistance, and Transwell was used to detect the effect of Smurf1 knockout on cell migration. Results HeLa and A549 cell lines with stable Smurf1 knockout were successfully constructed by interfering lentivirus system. Stable knock down Smurf1 inhibited the migration rate of the cells. Conclusion knockout of Smurf1 inhibits cell migration.
【作者单位】： 中国医学科学院医学实验动物研究所北京协和医学院比较医学中心;
【基金】：协和青年基金资助 中央高校基本科研业务费专项资金资助(3332016077) 中国医学科学院医学实验动物研究所基本科研业务费专项资助(2016ZX310033)
【分类号】：R329.2
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本文编号：1825330