不同品牌血清对小鼠骨髓基质细胞培养的影响

发布时间：2018-05-01 10:17

本文选题：血清 + 骨髓基质细胞　；参考：《现代预防医学》2017年05期

【摘要】：目的研究不同品牌血清对小鼠骨髓基质细胞体外培养的影响,以确保骨髓基质细胞体外培养的状态。方法通过倒置显微镜观察骨髓基质细胞在含不同血清培养基中的细胞形态,MTT法检测其生长趋势,AO/PI荧光染料检测细胞的凋亡情况,利用流式细胞法分析细胞周期分布情况。结果 NQBB血清培养的骨髓基质细胞更容易形成细胞集落且数量最多,细胞生长呈上升趋势;骨髓基质细胞在PAN、Ex Cell Bio、BI碳吸附、Gbico血清的连续培养过程中,细胞数目在不同程度上呈下降趋势;BI特级血清培养的细胞数目最少但在连续培养中呈上升趋势。在凋亡实验中,NQBB和BI特级血清培养的骨髓基质细胞凋亡率比Ex Cell Bio血清低。在细胞周期实验中,NQBB血清培养的骨髓基质细胞在G0/G1期的比例高于BI特级(P=0.040)和Ex Cell Bio血清(P=0.043)。结论 NQBB血清相比其它5种血清更适合骨髓基质细胞的体外培养。
[Abstract]:Objective to study the effect of different brands of serum on bone marrow stromal cells culture in vitro in order to ensure the status of bone marrow stromal cells in vitro. Methods the cell morphology of bone marrow stromal cells in different serum culture medium was observed by inverted microscope. The growth trend of bone marrow stromal cells was detected by MTT method. The apoptosis of bone marrow stromal cells was detected by AOP Pi fluorescent dye. The cell cycle distribution was analyzed by flow cytometry. Results the bone marrow stromal cells cultured with NQBB serum were more likely to form colony and the number of cells was the most, and the growth of bone marrow stromal cells was on the rise, and the bone marrow stromal cells were cultured in the continuous culture of PANEx Cell BioBI carbon adsorbed Gbico serum. The number of cells cultured with BI special serum was the least in different degrees, but increased in continuous culture. The apoptosis rate of bone marrow stromal cells cultured in NQBB and BI serum was lower than that in Ex Cell Bio serum. The ratio of bone marrow stromal cells cultured with NQBB serum in the cell cycle test was higher in G0/G1 phase than that in BI Bio serum (0.040) and Ex Cell Bio serum (P < 0.043). Conclusion NQBB serum is more suitable for in vitro culture of bone marrow stromal cells than other five sera.
【作者单位】：广东药科大学生命科学与生物制药学院/广东省生物技术候选药物研究重点实验室;广东药科大学药科学院;
【基金】：国家自然科学基金(81403317) 广东省学科建设专项资金项目(2013LYM 0044) 建设中医药强省科研课题(20141163)
【分类号】：R329.2

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