苦参总碱对溃疡性结肠炎模型大鼠的影响

发布时间：2018-05-02 11:44

本文选题：苦参总碱 + 溃疡性结肠炎　；参考：《中药药理与临床》2017年02期

【摘要】：目的:研究苦参总碱对溃疡性结肠炎模型大鼠的治疗作用及对核因子-κB、下游炎症细胞因子表达的影响。方法:采用TNBS(2,4,6-三硝基苯磺酸)+乙醇法制备大鼠溃疡性结肠炎(ulcerative colitis)模型,随机分为模型对照组、苦参总碱低、中、高剂量组(100、200、400 mg/kg)、阳性对照组(柳氮磺胺吡啶,Sulfasalazine)等5组,另设正常对照组大鼠10只。留取结肠组织标本,观察结肠大体形态变化,并做病理组织学检查。采用酶联免疫吸附测定法(enzyme-linked immunosorbent assay,ELISA)法观察溃疡性结肠炎模型大鼠血清中肿瘤坏死因子-α(TNF-α)、白介素-8(IL-8)和白介素-1(IL-1)的含量,采用Western blot法检测结肠组织NF-κB的表达水平。结果:与模型组相比,苦参总碱100 mg/kg组能改善溃疡性结肠炎模型大鼠结肠大体形态评分,减轻肠道炎性反应和粘膜损伤;苦参总碱(100、200、400 mg/kg)干预后能降低血清中炎症因子TNF-α、IL-1和IL-8的水平。苦参总碱治疗组均能下调结肠组织中NF-κB的蛋白表达,呈现的剂量依赖性。结论:苦参总碱能够抑制NF-κB的活化,减少炎性因子表达,对溃疡性结肠炎有明显的治疗作用。
[Abstract]:Aim: to study the therapeutic effect of total alkaloids of Sophora flavescens on ulcerative colitis model rats and its effect on the expression of nuclear factor- 魏 B and inflammatory cytokines downstream. Methods: the rat model of ulcerative colitis-induced ulcerative colitiswas was prepared by TNBS2O2C4-trinitrobenzene sulfonic acid (TNBSX) ethanol method. The rats were randomly divided into 5 groups: model control group, low total alkaloid of Sophora flavescens, middle and high dose group, 100200,400 mg / kg, positive control group (sulfasalazine), etc. In addition, 10 normal control rats were set up. The colonic tissue samples were collected and the colonic gross morphologic changes were observed and histopathological examination was made. The serum levels of tumor necrosis factor- 伪 (TNF- 伪), interleukin-8 (IL-8) and interleukin-1 (IL-1) in serum of rats with ulcerative colitis were measured by enzyme-linked immunosorbent assay-ELISA-ELISA. The expression of NF- 魏 B in colonic tissue was detected by Western blot method. Results: compared with the model group, the total alkaloid of Sophora flavescens 100 mg/kg group could improve the colonic gross morphologic score of rats with ulcerative colitis and alleviate the inflammatory reaction and mucosal injury. Total alkaloid of Sophora flavescens 100200 mg / kg decreased the levels of TNF- 伪 IL-1 and IL-8 in serum. The total alkaloids of Sophora flavescens could down-regulate the expression of NF- 魏 B in colonic tissue in a dose-dependent manner. Conclusion: the total alkaloids of Sophora flavescens can inhibit the activation of NF- 魏 B, reduce the expression of inflammatory factors, and have an obvious therapeutic effect on ulcerative colitis.
【作者单位】：四川大学华西医院消化内科;四川省人民医院消化内科;西南医科大学药学院;
【基金】：泸州市-西南医科大学联合基金(20130645)
【分类号】：R285.5;R-332

【相似文献】