不同健康状态对临床实验指标的影响及其与HLA-DQB1等位基因的关联

发布时间：2018-05-03 00:33

本文选题：一般健康问卷 + 血常规　；参考：《大连医科大学》2011年硕士论文

【摘要】：目的:本研究通过对不同人群一般健康状况的调查,以及临床实验指标的检测,探讨不同健康状态对临床实验指标变化的影响,并且在分子生物学水平上研究不同健康状态与HLA-DQB1等位基因的关联。旨在了解不同健康状态及个体差异对疾病的发生产生的影响。方法: 1.选取职业人群32人(司机14人,护士18人),运动员36人分别作为观察组,随机选取正常健康体检人员33人,作为对照组;高考人群26人,作为自身配对(备考期和恢复期),对以上人群统一发放一般健康问卷,测量其一般健康状况,并检测其血常规、血清生化及免疫指标。通过比较各组间量表的各项统计指标及各项实验室检测指标,探讨不同健康状态对临床实验指标变化的影响。 2.采用PCR技术检测职业人群和对照组人群的HLA-DQB1等位基因,分析两组之间HLA-DQB1等位基因分布频率的差异,并研究其与临床实验指标之间的关系。结果: 1.职业人群组和高考人群组量表的各项统计指标差异均具有统计学意义,而运动员组未发现量表的各项指标有统计学意义。 2.观察组和对照组之间某些血常规、血清生化及免疫指标的差异具有统计学意义(P0.05),相较于对照组,职业人群组嗜酸性粒细胞百分比(EO%)、血小板分布宽度(PDW)升高,血小板压积(PCT)、平均血小板体积(MPV)、血小板(PLT)降低,葡萄糖(GLU)、肌酐(Cre)、白蛋白(ALB)、球蛋白(Glob)、总蛋白(TP)、总胆固醇(Chol)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、超敏c反应蛋白(hs-CRP)升高,白球比(A/G)降低,免疫球蛋白A(IgA)、免疫球蛋白G(IgG)、补体C3、补体C4升高;运动员组血小板(PLT)、血小板压积(PCT)、淋巴细胞绝对值(LYMPH#)、嗜碱性粒细胞绝对值(BASO#)、嗜碱性粒细胞百分比(BASO%)、白蛋白(ALB)、葡萄糖(GLU)、肌酐(Cre)升高;高考前相较于高考后,葡萄糖(GLU)、高密度脂蛋白(HDL)升高,而肌酐(Cre)、低密度脂蛋白(LDL)、总蛋白(TP)、白蛋白(ALB)、球蛋白(Glob)降低。 3.职业人群组与对照组之间HLA-DQB1基因分布频率差异不具有统计学意义(P0.05);HLA-DQB1*02位点对职业人群组红细胞分布宽度系数(RDW-CV)的变化影响具有统计学意义,HLA-DQB1*06位点对职业人群组超敏c反应蛋白(hs-CRP)的变化影响具有统计学意义(P0.05);HLA-DQB1*02位点对对照组白球比(A/G)、球蛋白(Glob)、免疫球蛋白A(IgA)的影响具有统计学意义,HLA-DQB1*04位点对对照组嗜酸性粒细胞绝对值(EO#)、嗜酸性粒细胞百分比(EO%)的影响具有统计学意义,HLA-DQB1*06位点对对照组总胆固醇(Chol)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)的影响具有统计学意义(P0.05)。结论:不同健康状态对机体某些临床实验指标的变化有显著影响,并且这种变化可能与HLA-DQB1等位基因表型有关。
[Abstract]:Objective: to investigate the influence of different health status on the changes of clinical experimental indexes by investigating the general health status of different population and the detection of clinical experimental indexes. The association between different health status and HLA-DQB1 allele was studied at molecular biological level. The purpose of this study was to understand the effects of different health states and individual differences on the occurrence of disease. Methods: 1. 32 professional people (14 drivers, 18 nurses, 36 athletes) were selected as observation group, 33 healthy persons as control group, 26 college entrance examination group, As self-matched (preparation period and convalescence period), the general health questionnaire was issued to the above population, their general health status was measured, and their blood routine, serum biochemical and immune indexes were tested. The effects of different health states on the changes of clinical experimental indexes were studied by comparing the statistical indexes and laboratory indicators of the scale. 2. PCR technique was used to detect the HLA-DQB1 alleles in the occupational population and the control group. The difference of the distribution frequency of HLA-DQB1 alleles between the two groups was analyzed and the relationship between HLA-DQB1 alleles and clinical trial indexes was studied. Results: 1. The differences between the professional group and the college entrance examination group scale were statistically significant, while the athletes group did not find any statistical significance. 2. Compared with the control group, the percentage of eosinophilic granulocytes in the occupational group was increased, and the platelet distribution width was increased, compared with the control group, the difference of serum biochemical and immunological indexes was statistically significant between the observation group and the control group (P < 0.05), and compared with the control group, the percentage of eosinophilic granulocytes in the occupational group was increased. The mean platelet volume (MPV), the platelet count (PLT) were decreased, the glucose gluconate (GLU), creatinine (creatine), Albumin (ALB), globulin (globulin), total protein (TTP), total cholesterol (TC) Choln, high density lipoprotein (HDL), low density lipoprotein (LDL), hypersensitive c reactive protein (hs-CRP) increased, and the white ball ratio was decreased. Immunoglobulin A (IgA), immunoglobulin G (IGG), complement C 3, complement C 4 were increased in athletes group, platelets in the athletes group were increased by PLTN, platelet compaction (PCT), lymphocyte absolute value (LYMP), basic granulocyte (basophil), basophilic granulocyte (basophil), percentage of basophilic granulocytes (basophil), Albumin (Alb), glucose gluconate (GLU), creatinine (creatinine). Compared with those before and after the college entrance examination, the levels of glucose gluconium and high density lipoprotein (HDL) increased, while creatinine (creatinine), low density lipoprotein (LDL), total protein (TPN), Albumin (Alb), globulin (globulin) decreased. 3. There was no significant difference in the frequency distribution of HLA-DQB1 gene between the occupational group and the control group. There was no significant difference in the distribution frequency of HLA-DQB1 gene between the occupational group and the control group. There was no significant difference in the frequency of HLA-DQB1 gene distribution between the occupational group and the control group. The effect of C-reactive protein hs-CRP) on HLA-DQB1O02 locus in the control group was statistically significant. There was statistical significance in the effect of HLA-DQB1O02 locus on the eosinophil absolute value, eosinophilic granulocyte, eosinophilic granulocyte in the control group (A / P / G / G), globulin and immunoglobulin A / IgA). The effect of HLA-DQB1 / 06 locus on the total cholesterol Cholla, HDL and LDL in the control group was statistically significant (P 0.05). Conclusion: different health status has a significant effect on the changes of some clinical experimental indexes, and this change may be related to the allelic phenotype of HLA-DQB1.
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R346

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