不同取血方式及性别对成年Wistar大鼠11种血液生物化学指标的影响

发布时间：2018-05-04 08:54

本文选题：尾静脉 + 腹主动脉　；参考：《首都医科大学学报》2017年04期

【摘要】：目的探讨尾静脉、腹主动脉和下腔静脉取血对成年Wistar大鼠部分血液生物化学指标的影响。方法 34只成年Wistar大鼠按性别等分2组,各组麻醉后依次采集尾静脉,腹主动脉和下腔静脉血,血清用7180E型全自动生物化学分析仪测定11种血液生物化学指标,包括丙氨酸氨基转移酶(alanine aminotransferase,ALT)、门冬氨酸氨基转移酶(aspartate aminotransferase,AST)、尿素氮(urea nitrogen,BUN)、血糖(glucose,GLU)、总蛋白(total protein,TP)、白蛋白(albumin,ALB)、血肌酐(creatinine,Cr)、三酰甘油(triglyceride,TG)、总胆固醇(total cholesterol,TC)、高密度脂蛋白胆固醇(high density lipoproteincholesterol,HDL-C)、低密度脂蛋白胆固醇(low density lipoprotein-cholesterol,LDL-C)。结果在雌性Wistar大鼠中,尾静脉和下腔静脉血中GLU,TP和Cr差异有统计学意义(P0.05);尾静脉和腹主动脉血中GLU、TP、ALB、Cr、TG、TC和HDL-C差异有统计学意义(P0.05)。雄性Wistar大鼠中,尾静脉和下腔静脉血中ALT、AST、GLU、TP和Cr差异有统计学意义(P0.05);尾静脉和腹主动脉血中ALT、AST、GLU、TP、ALB、TG、TC、HDL-C和LDL-C差异有统计学意义(P0.05)。不同性别之间:尾静脉取血时AST、TC和LDL-C差异有统计学意义(P0.05);下腔静脉取血时TP、ALB、Cr、TG、TC和LDL-C差异有统计学意义(P0.05);腹主动脉取血时TP、ALB、Cr、TC和LDL-C差异有统计学意义(P0.05)。结论不同取血方式和性别会影响成年Wistar大鼠的血液生物化学指标。
[Abstract]:Objective to investigate the effects of caudal vein, abdominal aorta and inferior vena cava on some biochemical indexes in adult Wistar rats. Methods 34 adult Wistar rats were divided into two groups according to sex. The caudal vein, abdominal aorta and inferior vena cava were collected in turn after anesthesia. These include alanine aminotransferase (alt), aspartate aminotransferase (AST), urea nitrogen nitrogenase (BUNA), glucose (GLU), total protein (TP), albumin (ALB), serum creatinine (Cr), triglyceride (TG), total cholesterol (TC), high density cholesterol (HDL-C), low density cholesterol (HDL-C), low density cholesterol (HDL-C). Low density lipoprotein-cholesterol (LDL-C). Results in female Wistar rats, the differences of GLUTP and Cr in caudal vein and inferior vena cava were statistically significant (P 0.05), and there were significant differences in TGTC and HDL-C between caudal vein and abdominal aorta (P 0.05). In male Wistar rats, there was a statistically significant difference in the levels of alt, AST, GLU, TP and Cr between the caudal vein and inferior vena cava, and in the blood of the caudal vein and the abdominal aorta, there was a significant difference in HDL-C and LDL-C between the tail vein and the abdominal aorta. There were significant differences in TC and LDL-C in caudal vein, in inferior vena cava, in TGG and LDL-C in caudal vein, and in LDL-C and LDL-C in abdominal aorta. Conclusion Blood biochemical indexes of adult Wistar rats are affected by different blood sampling methods and sex.
【作者单位】：首都医科大学医学实验与测试中心;首都医科大学化学生物学与药学院;
【基金】：首都医科大学校长基金技术类(2015JS29)~~
【分类号】：R-33

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