肥胖对糖尿病模型大鼠血清胰高血糖样肽-1表达的影响

发布时间：2018-05-05 20:44

本文选题：肥胖 + 糖尿病　；参考：《中国老年学杂志》2017年11期

【摘要】：目的探讨肥胖对糖尿病大鼠血清胰高血糖样肽(GLP)-1表达的影响。方法选取60只雄性大鼠,随机选取20只给予正常喂养纳入对照组,其余40只给予链脲菌素30 mg/kg制成糖尿病大鼠模型,按照随机数字表法分为两组,每组各20只,分别给予普通饲料以及高脂喂养12 w计算Lee指数,并纳入肥胖组及非肥胖组。应用酶联免疫法(ELISA)分别测定三组大鼠血清GLP-1、白细胞介素(IL)-18以及肿瘤坏死因子(TNF)-α、甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C)水平,分析血清GLP-1水平与IL-18、TNF-α相关性。结果肥胖组大鼠Lee指数、空腹血糖(FBG)、TG、TC、LDL-C、IL-18、TNF-α显著高于非肥胖组和对照组大鼠,而血清GLP-1、HDL-C显著低于非肥胖组和对照组大鼠;非肥胖组大鼠Lee指数、FBG、TG、TC、LDL-C、IL-18、TNF-α显著高于对照组大鼠,GLP-1、HDL-C显著低于对照组大鼠(P0.05);Spearman相关性分析可知,大鼠血清GLP-1水平与Lee指数、FBG、IL-18、TNF-α均呈显著负相关(P0.05)。结论肥胖会显著降低大鼠血清GLP-1表达,而随着IL-18、TNF-α水平的升高,血清GLP-1水平逐渐降低,为预防及治疗糖尿病提供了新的思路。
[Abstract]:Objective to investigate the effect of obesity on the expression of serum glucagon like peptide (GLPn-1) in diabetic rats. Methods Sixty male rats were randomly divided into two groups: control group (n = 20), normal feeding group (n = 20) and streptozotocin group (n = 40) treated with streptozotocin for 30 mg/kg. The Lee index was calculated by normal diet and high fat feeding for 12 weeks, and was included in obesity group and non obese group. The levels of GLP-1, IL-IL-18, TNF- 伪, TGG, TCC, LDL-C and HDL-C in serum of three groups of rats were measured by enzyme linked immunosorbent assay (Elisa). The correlation between serum GLP-1 level and IL-18 TNF- 伪 was analyzed. Results the Lee index and fasting blood glucose of obese rats were significantly higher than those of non-obese group and control group, while the serum GLP-1 HDL-C level was significantly lower than that of non-obese group and control group. The correlation analysis of Lee index (Lee index) and the level of IL-18TNF- 伪 in the control group was significantly higher than that in the control group (P < 0.05). The results showed that the level of serum GLP-1 was negatively correlated with the Lee index and the level of IL-18TNF- 伪 in the control group (P 0.05). Conclusion Obesity can significantly decrease the expression of GLP-1 in serum of rats, but with the increase of IL-18 TNF- 伪, the level of serum GLP-1 decreases gradually, which provides a new idea for the prevention and treatment of diabetes.
【作者单位】：郑州人民医院内分泌科;
【分类号】：R-332;R587.1

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