三种心脏骤停大鼠模型心肺复苏效果及脑损伤程度的研究

发布时间：2018-05-06 09:04

本文选题：大鼠 + 心肺复苏　；参考：《中南大学》2012年硕士论文

【摘要】：目的：对比三种不同方法致大鼠心脏骤停后心肺复苏的效果,并测定复苏成功后不同时间点(3小时、6小时、12小时)大脑皮层组织的ICAM-1、VCAM-1的表达,从而判断复苏后的脑损伤情况,为临床心肺脑复苏研究提供理论依据。方法：利用窒息、3千克重物压迫胸部、夹闭心底血管丛三种方法制造大鼠心脏骤停动物模型,每组分别选择复苏成功的大鼠15只,分别在3小时、6小时、12小时同法处死,每个时间点5只,取大脑皮层组织用免疫组化方法测定ICAM-1、VCAM-1的蛋白表达,复苏过程中持续监测血压、心率变化,记录心脏骤停的时间(TCA)和自主循环恢复的时间(TROSC)。结果：(1)复苏前各组HR、BP对比无差异(P0.05),致CA所需时间窒息组较另两组短(P0.05),自主循环恢复时间各组对比无差异(P0.05),窒息组复苏成功率为77%,3千克重物压迫胸部组为72%,夹闭心底血管丛组为72%,差异无统计学意义(P0.05)。 (2)与对照组比较,实验组各组各相应时间点的ICAM-1和VCAM-1的表达均明显增高(P0.05),但各实验组之间ICAM-1和VCAM-1的表达差异不显著(P0.05)。3Kg重物组6h和12h脑组织ICAM-1和VCAM-1含量较3h高(P0.05),夹血管组6h和12hICAM-1表达较3h高(P0.05)。结论：三种心肺复苏动物模型均可取,复苏成功率各组无差异,但窒息法和3千克重物压迫胸部法操作简单,稳定性更好。三种不同方法所致的大鼠脑损伤的程度基本一致。三种模型均可用于今后的心肺复苏后脑损伤的研究。
[Abstract]:Objective: to compare the effects of three different methods on cardiopulmonary resuscitation (CPR) after cardiac arrest in rats, and to determine the expression of ICAM-1 and VCAM-1 in cerebral cortex at different time points after resuscitation, so as to judge the brain injury after resuscitation. To provide theoretical basis for clinical cardiopulmonary cerebral resuscitation. Methods: rat models of cardiac arrest were made by pressing the chest with 3 kg of weight of asphyxia and closing the vascular plexus in the bottom of the heart. 15 rats with successful resuscitation were selected for each group, and the rats were killed in the same way at 3 hours, 6 hours and 12 hours respectively. The expression of ICAM-1 VCAM-1 protein was determined by immunohistochemical method. The changes of blood pressure, heart rate, the time of cardiac arrest and the recovery time of autonomic circulation were continuously monitored during resuscitation. Results before resuscitation, there was no significant difference in HRBP in each group (P 0.05). The time required to cause CA in asphyxia group was shorter than that in the other two groups (P 0.05). There was no difference in the recovery time of spontaneous circulation in each group (P 0.05). The success rate of resuscitation in asphyxia group was 77 kg and 72b in chest compression group. In the basal vascular plexus group, there was no significant difference between the two groups (P 0.05). (2) compared with the control group, The expression of ICAM-1 and VCAM-1 in the experimental group was significantly higher than that in the control group at the corresponding time points, but there was no significant difference in the expression of ICAM-1 and VCAM-1 among the experimental groups. The contents of ICAM-1 and VCAM-1 in brain tissue in the heavy body group at 6 and 12 hours were higher than those in the control group at 3 hours, and the expression of 12hICAM-1 in the vascular clamp group at 6 hours and 3 hours was higher than that in the vascular clamp group at 6 hours and 3 hours. Conclusion: all three animal models of cardiopulmonary resuscitation have no difference in the success rate of resuscitation, but the asphyxia method and 3 kg weight compression chest method are simple and stable. The degree of brain injury induced by three different methods was basically the same. All three models can be used to study brain injury after cardiopulmonary resuscitation (CPR).
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R-332;R459.7

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