三七总皂苷与比索洛尔对心梗后左室重构大鼠心功能作用

发布时间：2018-05-06 18:41

本文选题：三七总皂苷 + 比索洛尔　；参考：《广州中医药大学》2012年硕士论文

【摘要】：背景：三七是五加科植物三七的干燥根,它的主要活性成分三七总皂苷(PNS)在块根中含量可达到12%。课题组前期研究证实PNS可激活血管紧张素转换酶2(ACE2)而抑制血管紧张素生成、肿瘤坏死因子α (TNF-α)的表达与基质金属蛋白酶2(MMP-2)的活化,显著改善急性心肌梗塞(AMI)病鼠左室心肌细胞形态结构的病理损伤及心脏指数,提高左室收缩和舒张功能,减轻其心肌缺血再灌注损伤,具有改善心梗后左室重构(LVRM)的作用。比索洛尔为第二代选择性β1-肾上腺素能受体阻滞剂,可阻止心梗后心脏功能的下降,改善并逆转左心室肥厚,改善心脏收缩功能,降低病死率。目的：采用结扎左冠状动脉前降支建立大鼠AMI模型,比较PNS和比索洛尔对病鼠急性心肌缺血心功能的改善作用,并探讨其应用于治疗心梗后左室重构的可行方案。方法：除假手术组外采用结扎大鼠左冠状动脉前降支的方法建立AMI模型,术后24h后随机分为正常对照组和实验组,连续4周分别灌胃给予生理盐水2mg·kg、比索洛尔25mg·kg-1和PNS40、80、120mg·kg-1,观察PNS和比索洛尔对病鼠室间隔舒张末期厚度(IVSd)、左室舒张末期内径(宽度)(LVIDJ)、室间隔收缩末期厚度(IVSs)、左室收缩末期内径(宽度)(LVIDs)、左室后壁舒张末期厚度(LVPWd)、左室后壁收缩末期厚度(LVPWs)、左室射血分数(EF)、左室收缩百分率(FS)、二尖瓣口舒张早期血流速度(MV)、心率(HR)等反映心功能变化指标的影响。结果：PNS高、中剂量组与比索洛尔组的MV、EF均明显上升(P0.01),中剂量PNS组及比索洛尔组的FS明显上升、LVIDd和LVIDs则减小(P0.01-0.05)。结论：PNS及比索洛尔均具有改善AMI后LVRM病鼠心功能的作用,可用于治疗心梗后的左室重构。
[Abstract]:Background: Panax notoginseng is the dried root of Panax notoginseng, and its main active component, Panax notoginseng saponins PNSs, can reach 12% in root tuber. Our previous studies have demonstrated that PNS can activate angiotensin converting enzyme (ACE2) and inhibit angiotensin production, tumor necrosis factor- 伪 (TNF- 伪) expression and matrix metalloproteinase-2 (MMP-2) activation. It can significantly improve the pathological damage and cardiac index of left ventricular myocardial cells in acute myocardial infarction (AMI) rats, improve the left ventricular systolic and diastolic function, alleviate the myocardial ischemia-reperfusion injury, and improve the left ventricular remodeling (LVRM) after myocardial infarction. Bisoprolol is a second generation selective 尾 _ 1-adrenergic receptor blocker, which can prevent the decline of cardiac function, improve and reverse left ventricular hypertrophy, improve cardiac systolic function and reduce mortality after myocardial infarction. Aim: to establish a rat AMI model by ligating the anterior descending branch of left coronary artery, and to compare the effects of PNS and Bisoprolol on the improvement of cardiac function in rats with acute myocardial ischemia, and to explore a feasible scheme for the treatment of left ventricular remodeling after myocardial infarction. Methods: the AMI model was established by ligating the anterior descending branch of left coronary artery in rats except the sham operation group. 24 hours after operation, the model was randomly divided into two groups: normal control group and experimental group. Normal saline 2mg kg, Bisoprolol 25mg kg-1 and PNS40 120mg kg-1 were administered intragastrically for 4 weeks. The effects of PNS and Bisoprolol on left ventricular septal end diastolic thickness, left ventricular end diastolic diameter (LVIDJ), interventricular septal end systolic thickness (IVSs) and left ventricular end systolic thickness (LVEL) in diseased rats were observed. The changes of cardiac function were influenced by the width of LVIDsI, left ventricular posterior diastolic thickness (LVPWdN), left ventricular posterior wall thickness (LVPWs1), left ventricular ejection fraction (EFN), left ventricular systolic percentage (FSN), mitral orifice early diastolic velocity (MVV), heart rate (HRR) and so on. Results in the middle dose group and the Bisoprolol group, the MVV EF was significantly higher than that in the middle dose group and the Bisoprolol group, while the FS of the middle dose PNS group and the Bisoprolol group were significantly increased. The levels of LVIDd and LVIDs in the middle dose PNS group and Bisoprolol group were decreased. Conclusion both AMI and Bisoprolol can improve the cardiac function of rats with LVRM's disease after AMI, and can be used in the treatment of left ventricular remodeling after myocardial infarction.
【学位授予单位】：广州中医药大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R96;R-332

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