黄明胶治疗大鼠慢传输型便秘

发布时间：2018-05-06 22:17

本文选题：黄明胶 + 慢传输型便秘　；参考：《中成药》2017年11期

【摘要】：目的探讨黄明胶对慢传输型便秘(slow transit constipation,STC)的治疗作用。方法健康SD大鼠120只按体质量随机分为分为空白组,模型组,阳性药组(麻仁丸,3 g/kg),黄明胶低、中、高剂量组(1、2、4 g/kg),每组20只,雌雄各半。空白组每天按体质量灌胃生理盐水,灌胃容积为10 m L/kg;模型组及各治疗组按体质量灌胃复方地酚诺酯混悬液,灌胃剂量为10 mg/kg,每天灌胃1次,连续28 d,建立大鼠慢传输型便秘模型。模型建立后,空白组与模型组灌胃生理盐水,治疗组分别以相应药物灌胃治疗,每天灌胃1次,各组动物每次容量均为10 m L/kg,治疗14 d。通过测定大鼠小肠炭末推进率、肠道Cajal细胞阳性表达面积以及结肠c-kit mRNA阳性表达率,考察便秘大鼠胃肠道蠕动性能。结果给予黄明胶中、高剂量治疗后,大鼠便秘症状得到显著改善,小肠推进率、肠道Cajal细胞阳性表达面积以及结肠c-kit mRNA阳性表达率与模型组相比,均存在显著性差异(P0.05)。结论黄明胶对STC治疗效果显著,中剂量即可有较好疗效。
[Abstract]:Objective to investigate the therapeutic effect of Huanggelatin on slow transit constipation of slow transit constipation. Methods 120 healthy Sprague-Dawley rats were randomly divided into blank group, model group, positive group (3 g 路kg ~ (-1) 路kg ~ (-1) of Maren pill, low, high dose group (1 / 2) and 4 g 路kg ~ (-1) 路kg ~ (-1) respectively, 20 rats in each group, half male and female. In the blank group, normal saline was given daily by body mass, the volume was 10 mL / kg, and the model group and the treatment group were given the compound diphenoxylate suspension by body mass, the dosage was 10 mg / kg, once a day. A rat model of slow transit constipation was established for 28 days. After the establishment of the model, the blank group and the model group were intragastrically administered with normal saline. The treatment group was treated with corresponding drugs once a day. The volume of each group was 10 mL / kg for 14 days. The peristalsis of gastrointestinal tract in constipation rats was investigated by measuring the propelling rate of small intestine charcoal, the area of positive expression of Cajal cells in intestine and the positive expression rate of c-kit mRNA in colon of rats with constipation. Results compared with the model group, the constipation symptoms were significantly improved, the intestinal propulsion rate, the positive area of intestinal Cajal cells and the positive rate of colonic c-kit mRNA expression were significantly improved after high dose treatment of Huanggelatin compared with the model group (P 0.05). Conclusion Yellow gelatin is effective in the treatment of STC.
【作者单位】：东阿阿胶股份有限公司研究院大产品研发部国家胶类中药工程技术研究中心;南京中医药大学药学院;
【基金】：山东省科技重大专项(2015ZDZX07001)
【分类号】：R285.5;R-332

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