睡眠剥夺对大鼠学习记忆和神经递质的影响及中西药的干预作用

发布时间：2018-05-07 19:32

本文选题：睡眠剥夺 + 对氯苯丙氨酸　；参考：《福建医科大学》2011年硕士论文

【摘要】：目的: 1.研究快速眼动睡眠剥夺(RSD)和对氯苯丙氨酸(PCPA)所致完全性失眠两种睡眠剥夺(SD)模型对大鼠学习记忆及血浆中单胺类神经递质去甲肾上腺素(NE)、多巴胺(DA)及5-羟色胺(5-HT)的含量的影响。 2.研究松郁安神方和阿普唑仑对两种SD模型干预后大鼠学习记忆及血浆中单胺类神经递质含量的变化。方法: 健康雄性Sprague-Dawley大鼠88只,体重200±10g,随机选取其中40只用于建立SD模型。采用改良多平台法(MMPM)建立RSD模型,采用腹腔注射PCPA建立完全性失眠模型,分成5组:空白对照(CC)组、大平台环境对照(TC)组、快速眼动睡眠剥夺(RSD)组、腹腔注射PCPA组、腹腔注射生理盐水(NS)对照组。另外48只用于药物干预:建立两种SD模型后分别用松郁安神方和阿普唑仑灌胃5天。大鼠分成6组:NS+蒸馏水组、PCPA+蒸馏水组、RSD+中药组、RSD+西药组、PCPA+中药组、PCPA+西药组。各组动物实验前后行Morris水迷宫测试大鼠的学习记忆能力。实验结束后,腹主动脉取血,用高效液相色谱-电化学检测法(HPLC-ECD)检测血浆中NE、DA、5-HT的含量。结果: 1.大鼠学习记忆能力变化: 造模后Morris水迷宫测试各组的潜伏期较造模前均下降(P0.05)。RSD造模后,RSD组的潜伏期比TC组、CC组明显延长(P0.05)。药物干预后,与RSD组相比,RSD+中药组和RSD+西药组大鼠潜伏期缩短(P0.05)。 2.血浆中NE、DA、5-HT含量的变化: (1)SD模型组:与CC组、TC组、NS组相比,RSD组、PCPA组NE含量升高(P0.05)。与CC组、PCPA组相比,RSD组DA含量下降,与NS组相比,PCPA组DA含量升高(P0.05)。与CC组、NS组比较,RSD、PCPA组5-HT含量下降(P0.05)。 (2)药物干预组:与RSD组、PCPA组、RSD+中药组相比,PCPA+中药组、PCPA+西药组NE含量下降;与RSD组比较,RSD+西药组NE含量下降;与PCPA组、PCPA+蒸馏水组比较,PCPA+中药组DA下降;与PCPA组、RSD组比较,RSD+西药组、PCPA+西药组、PCPA+中药组5-HT含量升高。结论: 1.RSD使大鼠的学习记忆能力受损。PCPA对学习记忆能力无显著影响。中药及西药灌胃均可使RSD大鼠学习记忆得到改善,提示药物干预能提高机体的学习记忆能力。 2.RSD使血浆中NE含量升高,DA、5-HT含量下降,PCPA失眠使血浆中NE、DA含量上升,5-HT含量下降。 3.中药干预后RSD大鼠血浆中5-HT含量升高,PCPA失眠大鼠NE、DA含量下降,5-HT含量升高。西药干预后RSD大鼠NE含量下降、5-HT含量上升, PCPA失眠大鼠5-HT含量上升。神经递质含量的变化提示药物干预可能通过改变失眠者体内的神经递质调节睡眠。
[Abstract]:Objective: 1. The effects of rapid eye movement sleep deprivation (RSDs) and complete insomnia induced by p-chlorphenylalanine (PCPA) on learning and memory and the contents of monoamine neurotransmitter norepinephrine (NE), dopamine (DA) and serotonin (5-HT) in plasma of rats were studied. 2. To study the changes of learning and memory and the content of monoamine neurotransmitters in plasma after the intervention of Songyu Anshen recipe and alprazolam on two SD models in rats. Methods: 88 healthy male Sprague-Dawley rats, weighing 200 卤10 g, were randomly selected to establish SD model. The RSD model was established by modified multi-platform method and the complete insomnia model was established by intraperitoneal injection of PCPA. The model was divided into five groups: blank control group, large platform environment control group, rapid eye movement sleep deprivation group, and intraperitoneal injection of PCPA group. The control group was treated by intraperitoneal injection of NS. The other 48 rats were used for drug intervention: two SD models were established and treated with Songyu Anshen recipe and alprazolam respectively for 5 days. Rats were divided into 6 groups: PCPA distilled water group and PCPA distilled water group. The learning and memory abilities of rats were tested by Morris water maze before and after the experiment. After the experiment, the blood was taken from the abdominal aorta and the plasma NEDAD 5-HT was detected by high performance liquid chromatography-electrochemical detection (HPLC-ECD). Results: 1. Changes of learning and memory ability in rats: The latency of Morris water labyrinth test group was significantly longer than that of TC group. After drug intervention, compared with RSD group, the latency of rats in RSD group and RSD group was shortened (P 0.05). 2. Changes of 5-HT in plasma: The content of NE in PCPA group was higher than that in TC group (CC group) and NS group (P 0.05). Compared with CC group, the DA content of PCPA group was lower than that of PCPA group, and the DA content of PCPA group was higher than that of NS group. Compared with CC group and NS group, the 5-HT content of RSDN PCPA group was decreased (P 0.05). (2) Drug intervention group: compared with RSD group, the content of NE in PCPA group was lower than that in RSD group, the NE content in western medicine group was lower than that in RSD group, and the DA level in PCPA group was lower than that in PCPA group. Compared with the PCPA group, the content of 5-HT in the PCPA group was higher than that in the western medicine group. Conclusion: 1.RSD had no significant effect on learning and memory ability. Both Chinese medicine and western medicine could improve the learning and memory of RSD rats, suggesting that drug intervention could improve the ability of learning and memory. The content of NE in plasma was increased by 2.RSD and the content of 5-HT in plasma was decreased. The content of NEDA in plasma was increased and the content of 5-HT in plasma was decreased. 3. The content of 5-HT in plasma of RSD rats was increased after the intervention of traditional Chinese medicine. The content of NEDA in rats with RSD insomnia decreased and the content of 5-HT increased. After intervention of western medicine, NE content decreased and 5 HT content increased in RSD rats, and 5-HT content increased in PCPA insomnia rats. Changes in neurotransmitters suggest that drug intervention may regulate sleep by altering neurotransmitters in insomnia patients.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R363

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