桂枝茯苓丸联用顺铂紫杉醇化疗提高卵巢癌多药耐药模型裸鼠生存率

发布时间：2018-05-09 00:43

本文选题：桂枝茯苓丸 + 卵巢癌　；参考：《科学技术与工程》2016年20期

【摘要】：研究桂枝茯苓丸逆转人卵巢癌耐顺铂细胞株(SKOV3/DDP)肿瘤模型的耐药作用及其对荷瘤BALB/c裸鼠生存率的影响,探讨其可能的作用机制。采用Cell Counting Kit-8试剂盒检测SKOV3/DDP对顺铂和紫杉醇的耐药性;采用SKOV3/DDP细胞建立卵巢癌耐药肿瘤模型,模型BALB/c裸鼠随机分为桂枝茯苓丸浓缩液16 g·kg~(-1)·d~(-1)(高)、8 g·kg~(-1)·d~(-1)(中)、4g·kg~(-1)·d~(-1)(低)剂量(中药组)、顺铂/紫杉醇组(化疗组)、低剂量桂枝茯苓丸与化疗联用组(中西联用组)和空白对照组,检测各处理组的抑瘤率。观察各组BALB/c裸鼠的生存情况;并采用荧光定量PCR检测各组肿瘤组织中MDR1 mRNA的相对表达。结果是SKOV3/DDP细胞对顺铂和紫杉醇的耐药倍数分别为4.13倍和3.85倍。随着桂枝茯苓丸浓缩液剂量的增加,抑瘤作用增强,裸鼠生存率提高,肿瘤组织MDR1 mRNA表达相对于对照组逐渐下降。西药化疗组在耐药模型裸鼠中的抑瘤效果高于中药组,中西药联用后抑瘤效果显著高于西药化疗组;与其他各组相比,西药化疗组的裸鼠平均体重明显下降,差异均具有显著性(P0.05,P0.01)。中西联用组裸鼠生存率高于桂枝茯苓丸低剂量组和西药化疗组。西药化疗组MDR1mRNA表达相对于对照组显著增加,中西药联用组MDR1 mRNA表达相对于对照组显著下降,差异均具有显著性(P0.05,P0.01)。说明桂枝茯苓丸能够逆转SKOV3/DDP耐药性卵巢癌模型裸鼠的耐药性,提高裸鼠生存率,其机制可能与抑制MDR1 mRNA表达有关。
[Abstract]:To study the effect of Guizhi Fuling pill on reversing the drug-resistance of human ovarian cancer cell line SKOV3 / DDP and its effect on the survival rate of nude mice bearing tumor BALB/c, and to explore its possible mechanism. The drug resistance of SKOV3/DDP to cisplatin and paclitaxel was detected by Cell Counting Kit-8 kit, and the drug-resistant tumor model of ovarian cancer was established by SKOV3/DDP cells. Model BALB/c nude mice were randomly divided into two groups: Guizhi Fuling Pill concentrated liquid (16g / kg ~ (-1) ~ (1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) (Chinese medicine group), Cisplatin / paclitaxel group (chemotherapy group), low dose Guizhi Fuling Pill combined with chemotherapy group (combined with Chinese and western medicine) and blank control group (P < 0.05), and the model mice were divided into three groups: Cisplatin / paclitaxel group (chemotherapy group) and blank control group. The tumor inhibition rate of each treatment group was detected. The survival of each group of BALB/c nude mice was observed, and the relative expression of MDR1 mRNA in tumor tissues was detected by fluorescence quantitative PCR. The results showed that the resistance of SKOV3/DDP cells to cisplatin and paclitaxel was 4.13 and 3.85 times respectively. With the increase of the concentration of Guizhi Fuling Pill, the anti-tumor effect was enhanced, the survival rate of nude mice was increased, and the expression of MDR1 mRNA in tumor tissue was gradually decreased compared with the control group. The anti-tumor effect of the western medicine chemotherapy group was higher than that of the traditional Chinese medicine group, and that of the western medicine chemotherapy group was significantly higher than that of the western medicine chemotherapy group, and compared with other groups, the average weight of the nude mice in the western medicine chemotherapy group was significantly lower than that in the western medicine chemotherapy group. The difference was significant (P 0.05, P 0.01). The survival rate of nude mice in combination group was higher than that of Guizhi Fuling pill group and western medicine chemotherapy group. Compared with the control group, the expression of MDR1mRNA in the western medicine chemotherapy group was significantly higher than that in the control group, and the expression of MDR1 mRNA in the combination of Chinese and western medicine group was significantly lower than that in the control group. The results indicated that Guizhi Fuling pills could reverse the drug resistance of SKOV3/DDP resistant ovarian cancer model and improve the survival rate of nude mice. The mechanism of Guizhi Fuling pills might be related to the inhibition of MDR1 mRNA expression.
【作者单位】：南阳理工学院张仲景国医国药学院;
【基金】：河南省科技厅基础与前沿项目(152300410179)资助
【分类号】：R737.31;R-332

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