肉苁蓉颗粒剂对帕金森病大鼠模型黑质纹状体多巴胺能神经元的保护作用研究

发布时间：2018-05-10 20:35

本文选题：肉苁蓉颗粒 + 帕金森病大鼠模型　；参考：《中华中医药学刊》2016年12期

【摘要】：目的:探讨肉苁蓉颗粒剂对帕金森病大鼠模型黑质纹状体多巴胺能神经元的保护作用。方法:采用6-羟基多巴胺单侧双靶点颅内定向注射法制备帕金森病大鼠模型,3周后据阿朴吗啡诱导的不自主运动,筛选造模成功大鼠,随机分为空白组,模型组,假手术组,肉苁蓉颗粒剂低剂量组(1 g·kg-1·d-1)和肉苁蓉颗粒剂高剂量组(3 g·kg-1·d-1),灌胃相应药物共4周。记录阿朴吗啡诱导的大鼠向健侧旋转的圈数;采用免疫组化研究观察黑质内酪氨酸羟化酶(TH)的表达;和免疫印迹法检测(Western-blot)观察纹状体内α-突出核蛋白(α-synuclein)、酪氨酸羟化酶(TH)、多巴胺转运蛋白(DAT)的表达;高效液相色谱-质谱联用(HPLC-MS/MS)法检测定大鼠纹状体多巴胺(DA)、3,4-二羟基苯乙酸(DOPAC)、高香草酸(HVA)的含量。结果:用药4周后,肉苁蓉组向健侧旋转的圈数与模型组比较明显减少(P0.05),黑质内TH阳性细胞较模型组明显增多,纹状体内α-synuclein表达下调、TH、DAT表达上调。纹状体DA、DOPAC含量明显升高(P0.05或P0.01)。高剂量组效果优于低剂量组。结论:肉苁蓉颗粒对帕金森病大鼠模型黑质和纹状体的多巴胺能神经元具有神经保护作用。
[Abstract]:Aim: to investigate the protective effect of Cistanche deserticola granules on dopaminergic neurons in substantia nigra striatum of Parkinson's disease rats. Methods: a rat model of Parkinson's disease was established by intracranial injection of 6-hydroxydopamine (6-hydroxydopamine) at one side and two targets. After 3 weeks' involuntary exercise induced by apomorphine, successful rats were selected and randomly divided into three groups: blank group, model group and sham operation group. Cistanche deserticola granules low dose group (1 g kg-1 d-1) and Cistanche granule high dose group (3 g kg-1 d -1) were gavaged for 4 weeks. The number of cycles of contralateral rotation induced by apomorphine was recorded, and the expression of tyrosine hydroxylase (THH) in substantia nigra was observed by immunohistochemistry. The expression of 伪 -synuclein (tyrosine hydroxylase) in striatum was detected by Western blot. High performance liquid chromatography-mass spectrometry (HPLC-MS / MS) method was used to determine the content of dopamine Dachlorine 3, 4-dihydroxyphenylacetic acid (DOPA) in the striatum of rats. Results: after 4 weeks of administration, the number of rotation circles in the contralateral direction of Cistanche deserticola group was significantly lower than that in the model group, the number of th positive cells in the substantia nigra was significantly higher than that in the model group, and the expression of 伪 -synuclein was down-regulated in the striatum. The content of DOPAC in striatum was significantly higher than that in control group (P 0.05 or P 0.01). The effect of high dose group was better than that of low dose group. Conclusion: Cistanche deserticola granule has neuroprotective effect on dopaminergic neurons in substantia nigra and striatum of Parkinson's disease rats.
【作者单位】：南京市中医院脑病科;南京市中医院中心实验室;南京市中医院中药研究所;
【基金】：江苏省中医药局科技项目(YB2015060) 南京市科技计划项目(201605036) 南京市中医院院内科研基金项目(YJLC201606)
【分类号】：R285.5;R-332

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