一种改良膝关节痛风性关节炎兔模型

发布时间：2018-05-11 03:16

本文选题：关节炎 + 膝关节　；参考：《中国组织工程研究》2017年24期

【摘要】：背景:良好的动物模型是研究痛风性关节炎重要的实验方法和手段。然而,目前尚无十分理想的痛风性关节炎动物模型,这给寻找新的药物、方法治疗痛风性关节炎带来阻碍。目的:评价改良后的兔膝关节痛风模型的有效性。方法:选取新西兰大白兔随机分成4组。空白对照组不做任何处理,右膝作为观察侧;假手术组取大网膜,处理后不包裹单钠尿酸盐结晶,单纯将大网膜植入兔右侧膝关节髌上囊腔;经典模型组将单钠尿酸盐结晶混悬液(0.1 g/L)注射入兔右侧膝关节髌上囊腔;改良模型组取大网膜,处理后包裹单钠尿酸盐结晶(100 mg/kg),植入兔右侧膝关节髌上囊腔。观察造模后1,2,3 d及造模后1,2,3,4周关节肿胀程度、活动情况及关节炎症反应等来评估改良模型的优缺点。结果与结论:(1)造模后4周内各个时间点改良模型组的兔关节功能均显著降低(P0.05);(2)经典模型组和改良模型组造模后关节肿胀指数分别在造模后第1或2天达到高峰,其后均开始下降,而改良模型组较经典模型组下降缓慢,造模后第4周时与空白对照组相比存在显著性差异;(3)大体观察:改良模型组和经典模型组均呈现出明显的关节炎症改变:滑膜广泛增生,伴充血水肿;关节腔内有较多关节液形成;周围组织充血水肿;软骨未见明显破坏;(4)经典模型组和改良模型组造模后血清和关节液中白细胞介素6及白细胞介素1β水平分别在术后第1和第2天达到高峰,其后均开始下降,改良模型组下降较经典模型组缓慢,至造模后4周时仍高于其他3组(P0.05);(5)结果说明,通过向兔膝关节腔内植入用自体大网膜包裹的单钠尿酸盐结晶可以成功地制造兔膝关节痛风性关节炎的模型,模型可维持至4周以上,较经典模型维持时间显著延长。
[Abstract]:Background: good animal models are important experimental methods and means to study gouty arthritis. However, there is no ideal animal model of gouty arthritis, which hinders the search for new drugs to treat gouty arthritis. Objective: to evaluate the effectiveness of modified rabbit knee joint gout model. Methods: new Zealand white rabbits were randomly divided into 4 groups. The blank control group did not do any treatment, the right knee was used as the observation side, the sham-operation group took the greater omentum, and after the treatment, the greater omentum was implanted into the right superior patellar sac of the right knee without encapsulating the single sodium uric acid crystal. In the classical model group, 0.1 g / L monosodium uric acid crystal suspension was injected into the right superior patellar capsule of the rabbit knee, and the modified model group was treated with the greater omentum and encapsulated with 100 mg / kg monosodium uric acid crystals and implanted into the right superior patellar capsule of the knee joint. To evaluate the advantages and disadvantages of the modified model, the degree of joint swelling, the activity and the reaction of arthritis were observed on the 3rd day after the establishment of the model and the 3th week after the establishment of the model. Results and conclusion the articular function of the modified model group was significantly decreased at each time point within 4 weeks after the establishment of the model. The joint swelling index of the classical model group and the modified model group reached the peak on the 1st and 2nd day after modeling, respectively. After that, both of them began to decrease, while the modified model group decreased more slowly than the classical model group. There was significant difference between the model group and the blank control group at the 4th week after modeling. The general observation showed that both the modified model group and the classical model group showed obvious changes of arthritis: extensive synovial hyperplasia with hyperemia and edema; There were more joint fluid formation in articular cavity, hyperemia and edema in surrounding tissue; The levels of interleukin 6 and interleukin 1 尾 in serum and joint fluid in the classical model group and the modified model group reached their peak on the first and second day after operation, and then began to decrease. The decline of the modified model group was slower than that of the classical model group, and was still higher than that of the other three groups at 4 weeks after the establishment of the model. The rabbit model of knee joint gouty arthritis can be successfully made by implantation of mononaturate crystals wrapped in autologous greater omentum into the rabbit knee joint cavity. The model can be maintained for more than 4 weeks, and the maintenance time of the model is significantly longer than that of the classical model.
【作者单位】：四川大学华西医院骨科运动医学中心;
【基金】：四川省科技厅支撑计划(2013SZ0019)~~
【分类号】：R-332;R589.7

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