感觉神经递质降钙素基因相关肽对去势大鼠骨髓间充质干细胞增殖及成骨能力的影响

发布时间：2018-05-12 19:23

本文选题：干细胞 + 骨髓干细胞　；参考：《中国组织工程研究》2017年13期

【摘要】：背景:研究发现降钙素基因相关肽能够增强成骨细胞的生物学活性,实验拟进一步检测降钙素基因相关肽对骨髓间充质干细胞成骨分化的影响。目的:探讨外源性降钙素基因相关肽对去势大鼠骨髓间充质干细胞增殖、成骨能力的影响。方法:切除雌性SD大鼠双侧卵巢建立骨质疏松模型,并通过micro-CT验证。从假手术和去势大鼠股骨中提取骨髓间充质干细胞,培养液中添加不同浓度降钙素基因相关肽,MTT法检测细胞增殖能力。对骨髓间充质干细胞进行成骨诱导,在诱导液中添加不同浓度降钙素基因相关肽,成骨诱导7 d,采用Real time RT-PCR和Western blot检测成骨特异性蛋白Runx2,Osterix的表达,成骨诱导21 d进行茜素红染色。结果与结论:(1)切除雌性大鼠双侧卵巢可成功建立骨质疏松模型;(2)不同浓度的降钙素基因相关肽可增强去势大鼠骨髓间充质干细胞的增殖及成骨分化能力,且具有剂量依赖性;(3)结果表明,降钙素基因相关肽对去势大鼠骨髓间充质干细胞增殖及成骨分化能力有直接促进作用,从而有利于骨形成。
[Abstract]:Background: calcitonin gene-related peptide (CGRP) has been found to enhance the biological activity of osteoblasts. The effect of calcitonin gene-related peptide on osteogenic differentiation of bone marrow mesenchymal stem cells was studied. Aim: to investigate the effects of exogenous calcitonin gene related peptide (CGRP) on proliferation and osteogenesis of bone marrow mesenchymal stem cells (MSCs) in ovariectomized rats. Methods: Osteoporosis model was established by excision of bilateral ovaries of female SD rats and verified by micro-CT. Bone marrow mesenchymal stem cells (BMSCs) were extracted from sham-operated and ovariectomized rats femur. The proliferation of MSCs was detected by MTT assay with different concentrations of calcitonin gene-related peptide. Bone marrow mesenchymal stem cells (BMSCs) were induced by osteogenesis. Calcitonin gene-related peptide (CGRP) was added to the induction fluid for 7 days. Real time RT-PCR and Western blot were used to detect the expression of the osteoblast specific protein Runx2Osterix. Alizarin red staining was performed on 21 days after osteogenesis induction. Results and conclusion Osteoporosis model could be established successfully by removing female rat bilateral ovaries. Different concentrations of calcitonin gene-related peptide could enhance the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells in ovariectomized rats. The results showed that calcitonin gene-related peptide could directly promote the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells in ovariectomized rats.
【作者单位】：军事口腔医学国家重点实验室口腔疾病国家临床医学研究中心陕西省口腔疾病重点实验室第四军医大学口腔医院口腔外科;解放军兰州军区总医院颌面外科;
【基金】：国家自然科学基金(81271103) 陕西省社发攻关课题(2012K16-10-01)~~
【分类号】：R329.2

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