润燥灵汤对干燥综合征模型小鼠颌下腺影响的研究

发布时间：2018-05-14 13:13

本文选题：干燥综合征 + 动物实验　；参考：《中华中医药学刊》2017年11期

【摘要】：目的:探讨润燥灵汤对干燥综合征(SS)模型小鼠颌下腺指数和病理的影响。方法:将90只C57bl/6j小鼠随机分为空白组、模型组、润燥灵低、中、高剂量级和强的松对照组,每组各15只。除空白对照组外,其余各组采用免疫诱导法建立SS模型小鼠,从造模第5周开始予润燥灵高、中、低剂量和强的松灌胃给药,空白对照组和模型对照组灌服等量生理盐水,每日1次,连续用药4周后,断颈处死小鼠,分离颌下腺,检测颌下腺指数和病变化。结果:模型对照组小鼠颌下腺指数明显低于空白对照组。经过4周的干预治疗后,强的松对照组、润燥灵高、中剂量组小鼠颌下腺指数均有不同程度增加,与模型对照组比较有统计学差异(P0.05),与强的松对照组比较,润燥灵高剂量组小鼠颌下腺无显著差异(P0.05)。病理检测结果显示模型对照组颌下腺小叶结构消失,腺泡、腺管结构紊乱,细胞间可见浆细胞、淋巴细胞浸润。强的松对照组和润燥灵高剂量组颌下腺小叶结构基本存在,腺泡、导管分布均匀,未见明显淋巴细胞浸润。结论:中药润燥灵能够改善SS模型小鼠的饮水量,增加颌下腺指数,改善模型小鼠颌下腺淋巴细胞浸润等病理改变。
[Abstract]:Objective: to study the effect of Runzaoling decoction on submandibular gland index and pathology in Sjogren's syndrome (Sjogren's syndrome) mice. Methods: 90 C57bl/6j mice were randomly divided into blank group, model group, low, medium, high dose group and prednisone control group with 15 rats in each group. The SS model mice were established by immune induction in the other groups except the blank control group. From the 5th week of the model, Runzaoling was given orally at high, medium and low doses and prednisone. The blank control group and the model control group were given the same amount of normal saline. Once a day for 4 weeks, the mice were killed by cervical amputation, the submandibular gland was isolated, the index of submandibular gland and the changes of disease were detected. Results: the index of submandibular gland in model control group was significantly lower than that in blank control group. After 4 weeks of intervention, the index of submandibular gland in prednisone control group, Runzaoling high, middle dose group increased in varying degrees, compared with model control group, there was statistical difference (P 0.05), and compared with prednisone control group, there was a significant difference between prednisone control group and prednisone control group. There was no significant difference in submandibular gland in high dose group (P 0.05). Pathological examination showed that the structure of submandibular lobule disappeared, the structure of acinar and glandular duct was disordered, plasma cells and lymphocytes infiltrated between cells in model control group. In the prednisone control group and Runzaoling high dose group, the submandibular lobule structure was basically present, the acinar and ductus were evenly distributed, and no significant lymphocyte infiltration was observed. Conclusion: Runzaoling can improve the quantity of drinking water, increase the index of submandibular gland and improve the pathological changes of lymphocyte infiltration of submandibular gland in SS model mice.
【作者单位】：贵阳中医学院第二附属医院风湿免疫科;
【基金】：贵州省优秀青年科技人才培养专项项目[黔科合人字(2013)50号] 贵州省科技创新人才团队项目[黔科合人才团队(2013)4033]
【分类号】：R285.5;R-332

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