慢性胃炎病症结合模型大鼠与能量代谢的实验研究

发布时间：2018-05-14 17:20

本文选题：慢性胃炎 + 动物模型　；参考：《辽宁中医药大学》2011年硕士论文

【摘要】：目的: 1、探讨胃炎大鼠脾胃虚证、湿热证模型的造模方法 2、探讨不同证型胃炎大鼠在能量代谢方面的指标差异材料和方法: 选取40只清洁级SD大鼠,随机分成四组,脾胃湿热组,脾胃虚弱组,模型组,正常组。造模期间观察大鼠的毛色、食量、体重的变化。造模四周后,湿热组8只,脾胃虚弱组9只,模型组、正常组各10只。取胃黏膜和骨骼肌通过酶促反应和定磷方法,检测各个组别能量代谢指标ATP酶活性,比较相关性。取胃窦部光镜下通过HE染色,分析病理结果。结果: 骨骼肌和胃黏膜ATP酶活性测试表明:脾胃湿热组与正常组相比,P0.05,有统计学差异。脾胃虚弱组与正常组相比,P0.05,有统计学差异。模型组与正常组相比,P0.05,有统计学差异。胃窦部HE病理染色显示:正常组:大鼠黏膜上皮细胞排列整齐,腺体形态规则,大小一致,固有层偶见炎性细胞浸润。模空组:胃黏膜血管扩张充血,腺体形态尚规则,但固有层可见少量炎症细胞浸润。提示已有慢性胃炎的病理学改变。湿热组:胃黏膜缺损,脱落,坏死,固有层见大量炎症细胞浸润。脾虚组:胃黏膜缺损,脱落,坏死,固有层可见炎症细胞较湿热少一些。结论: 脾胃虚弱组数值明显偏低,表明处于长期处于脾胃虚弱状态的大鼠ATP合成减少,机体能量代谢减少。脾胃湿热组数值明显偏高,表明基础酶活力和能量代谢增高。胃窦部病理染色显示:各组炎症程度,脾胃是热组脾胃虚弱组模型组正常组。
[Abstract]:Objective: 1. To explore the method of making model of spleen and stomach deficiency syndrome and damp-heat syndrome in rats with gastritis. 2. To explore the difference of energy metabolism in rats with different types of gastritis. Materials and methods: Forty SD rats of clean grade were randomly divided into four groups: spleen and stomach damp-heat group, spleen and stomach weakness group, model group and normal group. The changes of color, food intake and body weight of the rats were observed during modeling. Four weeks later, there were 8 rats in damp-heat group, 9 in spleen and stomach weakness group, 10 in model group and 10 in normal group. Gastric mucosa and skeletal muscle were measured by enzymatic reaction and phosphorus fixation to detect the activity of ATP enzyme in each group of energy metabolism indexes. The pathological results of gastric antrum were analyzed by HE staining under light microscope. Results: The activity of ATP enzyme in skeletal muscle and gastric mucosa showed that there was significant difference between the spleen and stomach dampness heat group and the normal group (P 0.05). Compared with the normal group, the spleen and stomach weak group had significant difference (P 0.05). There was statistical difference between model group and normal group (P 0.05). The HE staining of gastric antrum showed that in normal group, the epithelial cells were arranged neatly, the glands were regular, the size was the same, and inflammatory cell infiltration was occasionally seen in the lamina propria. Model empty group: gastric mucosal vasodilation hyperemia, glandular shape is regular, but there are a few inflammatory cells infiltration in lamina propria. It suggests that there are pathological changes in chronic gastritis. Damp-heat group: gastric mucosal defect, abscission, necrosis, lamina propria a large number of inflammatory cell infiltration. Spleen deficiency group: gastric mucosal defect, shedding, necrosis, inflammatory cells visible in the lamina propria less than damp and heat. Conclusion: The value of spleen and stomach weakness group was significantly lower, which indicated that ATP synthesis and energy metabolism were decreased in rats with chronic weakness of spleen and stomach. The value of spleen and stomach damp-heat group was significantly higher than that of the control group, indicating that the activity of basic enzymes and energy metabolism were increased. The pathological staining of gastric antrum showed that the degree of inflammation in each group and spleen and stomach were normal group.
【学位授予单位】：辽宁中医药大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R-332

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