病理性攻击大鼠模型构建及效果评估

发布时间：2018-05-15 00:39

本文选题：大鼠 + 动物模型　；参考：《重庆医科大学》2012年硕士论文

【摘要】：目的建立与人病理性攻击病理过程、生化改变相似，实用性、重复性好的动物模型，为研究病理性攻击行为发生发展的病理生理机制提供适宜的模型。方法 1.大鼠病理性攻击行为模型构建的探索。雄性Wistar大鼠40只，随机分为病理性攻击模型组、普通攻击模型组和正常对照组3组，另设10只用于激惹居住鼠的入侵组。（1）普通攻击模型组：入侵一居住攻击实验（2）病理性攻击模型组：①去奖赏性挫败实验②激惹刺激实验（3）正常对照组与入侵组：群居正常饲养。 2.模型大鼠的效果评估，包括行为学检测和生物学检测 2.1行为学检测 2.11总体攻击行为检测：①搏斗次数②威胁次数③攀压次数，大鼠总体攻击行为次数=①+②+③。 2.12病理性攻击行为检测：①攻击潜伏期②危险部位攻击次数，主要包括头部、颈部及腹部；③对屈服行为的继续攻击次数。 2.13其他行为学实验：①旷场实验②糖水偏好测试③大鼠高架十字迷宫实验④嗅觉灵敏度测试。 2.2生物学检测：免疫组织化学方法分别检测大鼠大脑前额皮质、杏仁核及下丘脑部位的5-HT及c-fos的表达水平。结果 1.行为学检测结果①病理性攻击组的总体攻击次数为[(101.17±2.85)次]，与普通攻击组[(52.5±5.36)次]、正常对照组[(8.83±1.34)次]比较均存在显著性差异(P0.01)；②各组大鼠各行为指标与总分呈中一高度正相关(r=0.379-0.929)；③病理性攻击组在危险部位攻击次数、屈服后继续攻击与攻击／威胁比与普通攻击组比较存在显著性差异(P0.01)；④病理性攻击组存在空间认知能力下降，，但不表现抑郁、焦虑情绪和嗅觉障碍（PO.05)。而普通攻击组则存在明显抑郁情绪。 2.生物学检测结果：①病理性攻击组大脑前额皮质、下丘脑及杏仁核区5-HT表达较正常对照组显著降低[PFC(11.7±0.84)；HA（10.1±1.11）；AMD（12.5±0.72）；（P 0.01)]。②普通攻击组大脑前额皮质、下丘脑区5-HT表达较正常对照组显著增高[PFC(20.9±1.08)；HA（19.1±1.77）；AMD（22.5±2.08）；（P 0.05)]，但杏仁核区5-HT表达较正常对照组无显著差异性（P0.05)。③病理性攻击组大脑前额皮质、下丘脑及杏仁核区c-fos表达较正常对照组显著升高[PFC(32.2±3.45)；HA（21.7±2.58）；AMD（23.7±0.68）；（P 0.01)]。④普通攻击组大脑前额皮质、下丘脑区c-fos表达较正常对照组显著升高[PFC(18.8±2.07)；HA（10.7±1.01）；AMD（15.4±0.85）；（P 0.05)]，而杏仁核c-fos表达较正常对照组无显著差异性（P0.05)。结论 1.行为学指标满足病理性攻击模型的评定标准； 2.生物学指标满足病理性攻击模型的评定标准； 3.可以排除其他因素对模型特异性的干扰； 4.本实验建立的大鼠病理性攻击模型形成稳定，容易复制，可用于基础机制研究。
[Abstract]:objective
It provides a suitable model for the study of pathophysiological mechanisms for the development of pathological attacks by establishing a pathological process of pathological attack, which has similar biochemical changes, practical and reproducible animal models.
Method
1. rat model of pathological attack behavior model, 40 male Wistar rats were randomly divided into pathological attack model group, ordinary attack model group and normal control group 3 groups, another 10 were used in invading group of irritable living mice. (1) common attack model group: invasive one attack experiment (2) pathological attack model group: (1) to reward Sexual frustration test: stimulation test (3) normal control group and invasive group: live in normal group.
2. evaluation of the effectiveness of the model rats, including behavioral testing and biological detection.
2.1 behavioral test
2.11 overall aggression test: (1) the number of fighting times, the number of threats, the number of times of climbing, and the total number of attacks in rats = 1 + + + 3.
2.12 detection of pathological attack behavior: (1) the number of attacks in the latent period, mainly including the head, neck and abdomen, and the number of successive attacks on yield behavior.
2.13 other behavioral experiments: (1) open field experiment (2) sugar water preference test; rat elevated maze test (4) olfactory sensitivity test.
2.2 biological detection: immunohistochemical method was used to detect the expression levels of 5-HT and c-fos in the rat frontal cortex, amygdala and hypothalamus.
Result
The results of 1. behavior test were [(101.17 + 2.85) times] in the pathological attack group, and the normal control group [(52.5 + 5.36) times], and the normal control group [(8.83 + 1.34) times] had significant difference (P0.01). (2) the behavior indexes of each group were positively correlated with the total score (r=0.379-0.929); (3) pathological attack group There was a significant difference (P0.01) between succumb attack and attack / threat compared with that of ordinary attack group (P0.01). (4) there was a decline in spatial cognitive ability in pathological attack group, but no depression, anxiety and olfactory disorder (PO.05), while the common attack group had obvious depression.
2. biological detection results: (1) the expression of 5-HT in the frontal cortex of the pathological attack group, the hypothalamus and amygdala region was significantly lower than that of the normal control group [PFC (11.7 + 0.84), HA (10.1 + 1.11), AMD (12.5 + 0.72), (P 0.01)) (P 0.01)). (2) the expression of 5-HT in the hypothalamus of the normal attack group was significantly higher than that in the normal control group [PFC (20.9 + 1.08). HA (19.1 + 1.77); AMD (22.5 + 2.08); (P 0.05)], but there was no significant difference in the expression of 5-HT in the amygdala region compared with the normal control group (P0.05). (3) the expression of c-fos in the cerebral prefrontal cortex, hypothalamus and amygdala region was significantly higher than that in the normal control group [PFC (32.2 + 3.45); HA (21.7 + 2.58); AMD (23.7 + 0.68); (P 0.01)]. (P 0.01)]. 4) ordinary attack. The c-fos expression in the hypothalamus was significantly increased by [PFC (18.8 + 2.07), HA (10.7 + 1.01) and AMD (15.4 + 0.85), and (P 0.05)), but there was no significant difference in c-fos expression between the amygdala and the normal control group (P0.05).
conclusion
1. behavioral indexes meet the criteria of pathological attack model.
2. biological indicators meet the evaluation criteria of pathological attack models.
3., we can exclude the interference of other factors to the specificity of the model.
4. the pathological attack model established in this experiment is stable and easy to replicate, and can be used for basic mechanism research.

【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R-332

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