结核分枝杆菌中3-甲基腺嘌呤DNA转葡糖基酶的功能及其调控研究

发布时间：2018-05-15 19:10

本文选题：结核分枝杆菌 + Rv1688　；参考：《华中农业大学》2011年硕士论文

【摘要】：结核分枝杆菌(M. tuberculosis)是一种重要的传染性病原菌,能够感染人和多种动物引发结核病。已有研究表明,结核分枝杆菌具有极强的基因突变能力并产生耐药性,从而能够长期在宿主细胞内潜伏。针对目前结核分枝杆菌的基因突变和DNA修复机制不清,本课题初步探索了结核分枝杆菌的DNA修复蛋白3-甲基腺嘌呤DNA转葡糖基酶(Rv1688)的功能及调控机制,主要获得了如下结果： (1)通过细菌双杂交筛选鉴定了一批与Rv1688相互作用的基因,其中包括一个TetR类型的转录因子Rv0825c；(2)发现了Rv0825c能够刺激Rv1688切割受损DNA活性,初步证实了这种刺激作用是通过增强Rv1688的损伤DNA结合活性；(3)证实了这两个蛋白之间的相互作用在耻垢分枝杆菌中也是保守的。(4)证实了Rv0825c能够与Rv1688的启动子序列相互作用,因此Rv0825c可能也对Rv1688基因的表达有转录调控影响。分枝杆菌中的DNA转葡糖基酶的功能及其调控很少被研究,由于这一类基因在DNA切除修复中可能发挥关键作用,因此对于病原菌的基因突变和耐药性的产生有直接影响。本研究对结核分枝杆菌的此类酶的功能及其调控机制的研究将增强我们对结核耐药问题的理解,相关工作有待进一步深入。
[Abstract]:Mycobacterium tuberculosis (M. tuberculosism) is an important infectious pathogen that can cause tuberculosis in humans and many animals. Studies have shown that Mycobacterium tuberculosis has a strong ability to mutate genes and develop drug resistance, so that it can be latent in host cells for a long time. In view of the unclear gene mutation and DNA repair mechanism of Mycobacterium tuberculosis at present, the function and regulation mechanism of DNA repair protein Rv1688 (3-methyladenine DNA transglucase) of Mycobacterium tuberculosis were preliminarily explored. The main results were as follows: (1) A number of genes interacting with Rv1688, including a TetR type transcription factor Rv0825cm-2, were identified by bacterial two-hybrid screening. It was found that Rv0825c can stimulate the damaged DNA activity of Rv1688 cleavage. It is preliminarily confirmed that this stimulative effect is due to the enhancement of the DNA binding activity of Rv1688.) the interaction between the two proteins is also conserved in Mycobacterium smeareus.) Rv0825c can interact with the promoter sequence of Rv1688. Therefore, Rv0825c may also have a transcriptional regulatory effect on the expression of Rv1688 gene. The function and regulation of DNA glucosylase in mycobacterium have been seldom studied. Because this kind of gene may play a key role in DNA excision repair, it has a direct effect on gene mutation and drug resistance of pathogenic bacteria. The study on the function and regulatory mechanism of this enzyme in Mycobacterium tuberculosis will enhance our understanding of drug resistance.
【学位授予单位】：华中农业大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R346;S852.61

【共引文献】