黄芪及其不同有效部位干预失血性贫血小鼠模型的效应机制研究

发布时间：2018-05-16 03:42

本文选题：黄芪有效部位 + 失血性贫血　；参考：《辽宁中医药大学》2012年硕士论文

【摘要】：目的：通过借助失血性贫血小鼠模型,观察黄芪及其不同有效部位对失血性贫血的干预作用,探讨黄芪不同有效部位补血的差异性,并初步探讨其作用机制。材料与方法：健康SPF级BALB/C小鼠132只,雌雄各半。所有小鼠经眶静脉采血测量小鼠外周血红蛋白浓度,根据外周血红蛋白浓度随机将小鼠分为正常组、模型组、中药对照组、黄芪组、酮糖苷组、糖苷组、酮糖组、酮苷组、多糖组、黄酮组、皂苷组,每组12只,雌雄各半。常规适应性饲养3d后进行造模：除正常组外,各组小鼠分别于第1d、4d、7d进行眶静脉放血,放血量为0.5ml/20g小鼠(即25ml/kg),造模第7d测小鼠血红蛋白浓度较前减少20%为造模成功。造模第2d,正常组、模型组给予蒸馏水灌胃,各给药组分别灌胃相应药物,均按1ml/100g体重剂量给药,连续灌胃14d。末次灌胃24h后取材,检测小鼠外周血红细胞计数(RBC)、血红蛋白浓度(HB)、红细胞压积(Hct), ELISA法测定实验小鼠骨髓干细胞因子(SCF)浓度、血清特异转录因子(GATA-1)浓度、血清及肾组织匀浆液红细胞生成素(EPO)浓度。结果： 1.模型组小鼠RBC、HB以及Hct均明显降低,表明失血性贫血动物模型成功建立。 2.一般状态：各给药治疗组对失血性贫血所引起的小鼠乏力倦卧、扎堆、体型消瘦、饮食减少等症状均能改善。 3.外周血象改变：与模型组比较,各给药组均能明显升高实验小鼠HB、RBC、Hct,糖苷组能够明显升高实验小鼠外周HB浓度,差异有统计学意义(P0.05)。 4.与模型组比较,除皂苷组外各给药组均较模型组小鼠血清GATA-1浓度、骨髓SCF浓度明显升高,黄酮组均优于酮糖苷组、糖苷组、酮糖组、酮苷组、多糖组,黄芪组均明显优于酮糖苷组、多糖组,但酮糖组在升高血清GATA-1浓度方面优于多糖组,黄芪组在升高骨髓SCF浓度方面优于糖苷组,差异有统计学意义(P0.05)。 5.与模型组比较,黄芪组、酮糖苷组、糖苷组、黄酮组明显升高小鼠肾组织匀浆EPO浓度,其中尤以糖苷组效用最佳,差异有统计学意义(P0.05)：与模型组比较,除皂苷组外各给药组均明显升高小鼠血清EPO浓度,其中黄芪组效用最佳,差异有统计学意义(P0.05),酮糖、糖苷配伍均优于单一组分用药。结论： 1.黄芪及其不同有效部位对失血性贫血均具有纠正作用。 2.黄芪及其不同有效部位均可增加失血性贫血小鼠HB、RBC及Hct,多糖+皂苷可能是改善失血性贫血小鼠外周血象的最佳有效配伍组合。 3.除皂苷组外黄芪及其有效部位均可通过促进小鼠骨髓SCF、血GATA-1、血EPO、肾组织EPO浓度的升高来实现其补血作用,黄酮增加骨髓SCF、血GATA-1作用最佳,多糖+皂苷升高血清及肾组织EPO效果最佳。
[Abstract]:Objective: Through observing the intervention effect of astragalus and its effective parts on hemorrhagic anemia with the help of hemorrhagic anemia mouse model, the difference of reinforcing blood in different effective parts of Astragalus membranaceus was discussed, and the mechanism of its action was preliminarily discussed. Materials and methods: There were 132 healthy BALB/C mice of SPF grade, half male and half female. The peripheral hemoglobin concentration of all mice was measured by orbital vein. According to the peripheral hemoglobin concentration, mice were randomly divided into normal group, model group, Chinese medicine control group, astragalus group, ketoglucoside group, ketosugar group, ketoside group, and ketoside group. Polysaccharide group, flavonoid group, saponins group, 12 rats in each group, half male and female. After 3 days of conventional adaptive feeding, the mice in each group were treated with orbital vein bleeding on day 1 and day 4, respectively, except the normal group. The volume of blood was 25 ml / kg in 0.5ml/20g mice, and the hemoglobin concentration of mice was decreased by 20% on the 7th day after the model was established. The results showed that the hemoglobin concentration of the mice decreased by 20% on the 7th day. On the 2nd day, the normal group and the model group were given distilled water by gavage, and each group was given the corresponding drug by stomach respectively, which was given by 1ml/100g body weight for 14 days. The samples were collected 24 hours after the last gastric administration. The red blood cell count (RBC), hemoglobin concentration (HBN), hematocrit (HCT), bone marrow stem cell factor (SCF) and serum specific transcription factor (GATA-1) were measured by ELISA. Erythropoietin (EPO) concentration in serum and renal homogenate. Results: 1. The HB and Hct of mice in the model group were significantly decreased, which indicated that the animal model of hemorrhagic anemia was established successfully. 2. General state: the symptoms of hemorrhagic anemia in each treatment group were improved, such as fatigue and sleepiness, mass, weight loss, diet reduction and so on. 3. The changes of peripheral blood image: compared with the model group, each administration group could significantly increase the HBB RBCU HCT in the experimental mice, and the glycoside group could significantly increase the peripheral HB concentration of the experimental mice, the difference being statistically significant (P 0.05). 4. Compared with the model group, the serum GATA-1 concentration and bone marrow SCF concentration in each administration group except the saponins group were significantly higher than those in the model group. The flavonoid group was superior to ketoglucoside group, ketoside group and polysaccharide group. Astragalus group was superior to ketoglucoside group and polysaccharide group, but ketosugar group was superior to polysaccharide group in raising serum GATA-1 concentration, Astragalus group was superior to glycoside group in raising bone marrow SCF concentration, the difference was statistically significant (P 0.05). 5. Compared with the model group, Astragalus membranaceus group, ketoglucoside group and flavonoid group significantly increased the concentration of EPO in the renal homogenate of mice, especially in the glycoside group, and the difference was statistically significant (P 0.05). The concentration of serum EPO was significantly increased in each administration group except the saponins group. The effect of astragalus membranaceus group was the best, and the difference was statistically significant (P 0.05). The compatibility of keto sugar and glycoside was better than that of single component. Conclusion: 1. Astragalus membranaceus and its different effective parts can correct hemorrhagic anemia. 2. Astragalus membranaceus and its different effective parts could increase HBG RBC and HCT in hemorrhagic anemia mice. Polysaccharide saponins might be the best combination to improve peripheral blood picture of hemorrhagic anemia mice. 3. In addition to saponins group, Astragalus membranaceus and its effective parts could enhance the concentration of bone marrow SCF, blood GATA-1, blood EPO, renal tissue EPO to achieve the effect of tonifying their blood. Flavonoids could increase bone marrow SCF, and blood GATA-1 had the best effect. The effect of polysaccharide saponins on serum and renal EPO was the best.
【学位授予单位】：辽宁中医药大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R-332;R285.5

【参考文献】