归芪益元膏对重离子辐射损伤模型大鼠肺肾旁效应的影响

发布时间：2018-05-16 05:41

本文选题：归芪益元膏 + 辐射损伤　；参考：《解放军医学杂志》2017年08期

【摘要】：目的探讨归芪益元膏防治重离子(12C6+)束辐射肺肾旁效应的作用机制,为临床辐射损伤防护提供新的思路。方法健康雄性Wistar大鼠60只,随机分为正常对照组(NCG组)、单纯辐射组(SRG组)、归芪益元膏组(GOG组),每组20只。灌胃给药7d,12C6+束辐射右侧肺部造模,造模后48h处死,取大鼠左肺、左肾及右肾组织,ELISAlike法检测DNA甲基化率,HE染色观察组织病理学改变,免疫组织化学法检测Dnmt1、Dnmt3a、Dnmt3b的表达。结果与正常对照组比较,单纯辐射组大鼠DNA甲基化水平显著降低(P0.01);左肺有炎症,左右肾无异常;Dnmt1、Dnmt3a、Dnmt3b表达明显升高(P0.01)。与单纯辐射组比较,归芪益元膏组大鼠DNA甲基化水平升高(P0.01);左肺炎症减轻;Dnmt1、Dnmt3a、Dnmt3b表达降低(P0.01)。Dnmt1、Dnmt3a、Dnmt3b蛋白均在支气管黏膜上皮细胞、肾小管上皮细胞的胞质内表达,各组均可见;正常对照组呈浅褐色-褐色染色,为弱阳性表达;单纯辐射组呈褐色-棕色染色,为强阳性表达;归芪益元膏组呈浅褐色-棕褐色染色,为中等程度阳性表达。结论归芪益元膏可减轻12C6+束辐射造成的肺肾旁效应,其机制可能与调控DNA甲基化水平有关。
[Abstract]:Objective to explore the mechanism of Guiqi Yiyuan ointment in preventing and treating lung and kidney side effects of heavy ion 12C6 beam radiation, and to provide a new idea for the protection of clinical radiation injury. Methods Sixty healthy male Wistar rats were randomly divided into normal control group (n = 20), radiation group (n = 20) and Guiqi Yiyuan ointment group (n = 20). The right lung model was induced by intragastric administration of 12C6 beam on the 7th day. The left lung, left kidney and right kidney were sacrificed 48 hours later. The DNA methylation rate and the histopathological changes were detected by ELISA-like method, and the expression of Dnmt1Dnmt3aDnmt3b was detected by immunohistochemistry. Results compared with the normal control group, the DNA methylation level in the radiation alone group was significantly lower than that in the control group, while there was inflammation in the left lung, and the expression of Dnmt3a1 + Dnmt3b in the left and right kidneys was significantly higher than that in the control group (P 0.01). Compared with the radiation group, the DNA methylation level of rats in the Guiqi Yiyuan ointment group was increased (P 0.01), and the expression of Dnmt3a Dnmt3b was reduced in the left lung inflammation group, and the expression of Dnmt3b protein in the epithelial cells of bronchial mucosa and renal tubules were all observed in all the groups, and the expression of Dnmt3b protein was found in the epithelial cells of bronchial mucosa and renal tubules. The normal control group showed light brown-brown staining with weak positive expression; the radiation group showed brown-brown staining with strong positive expression; the Guiqi Yiyuan ointment group showed light brown-tan staining with moderate positive expression. Conclusion Guiqi Yiyuan ointment can reduce the pulmonary and renal effects induced by 12C6 beam radiation, and its mechanism may be related to the regulation of DNA methylation level.
【作者单位】：甘肃中医药大学博士后流动站;甘肃中医药大学敦煌医学与转化省部共建教育部重点实验室;甘肃中医药大学附属医院急诊科;
【基金】：国家自然科学基金(81460695) 中国博士后科学基金(2016M602887)~~
【分类号】：R285.5;R-332

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