西宁市猪小肠结肠炎耶尔森菌病原学研究

发布时间：2018-05-16 14:09

本文选题：生猪 + 小肠结肠炎耶尔森菌　；参考：《中国病原生物学杂志》2017年01期

【摘要】：目的了解西宁市屠宰场生猪小肠结肠炎耶尔森菌分布及病原学特征。方法从猪咽拭子和回盲部肠内容物标本中提取细菌基因组DNA,进行小肠结肠炎耶尔森菌保守基因foxA的PCR检测,对阳性标本进行细菌分离,阳性菌株进行血清学分型、生物分型和毒力基因检测。结果 400份标本PCR扩增foxA基因阳性72份,其中49份培养分离出小肠结肠炎耶尔森菌,检出率为12.25%(49/400);49株小肠结肠炎耶尔森菌经生化鉴定主要为3个生物型:1A型(占16.33%)、3型(占79.59%)和4型(占4.08%);血清型以O∶3、O∶5、O∶8、O∶9为主,其中O∶3血清型39株(占79.59%),O∶5血清型5株(占10.20%),O∶8血清型2株(占4.08%),O∶9血清型1株(占2.04%),血清不确定型2株(占4.08%)。毒力基因分布特征为ail+、ystA+、ystB-、yadA+、virF+占73.46%(36/49),ail+、ystA+、ystB-、yadA-、virF-占10.20%(5/49),其他毒力基因型占16.33%(8/49)。结论西宁市生猪携带的小肠结肠炎耶尔森菌主要为致病性菌株,应加强对本地生猪小肠结肠炎耶尔森菌的监测。
[Abstract]:Objective to investigate the distribution and pathogenic characteristics of Yersinia enterocolitica in slaughterhouses of Xining. Methods the bacterial genomic DNAs were extracted from pig pharyngeal swabs and ileocecal intestinal contents. The foxA of Yersinia enterocolitica was detected by PCR. The positive samples were isolated and serologically typed. Genotyping and virulence gene detection. Results there were 72 positive samples of foxA gene amplified by PCR, 49 of which were isolated from Yersinia enterocolitica. The positive rate of Yersinia enterocolitica was 12.25% / 400% / 49 strains of Yersinia enterocolitica were mainly identified by biochemical identification as three biological types: type 1A (16.33335A) and type 4 (4.0810); serotypes were mainly O: 3 / O5: 0: 8: 9. Among them, 39 strains of O: 3 serotype (79.59) and 5 strains of serotype O5 (10.20% O: 8 serotype 2) (4.08% O: 9 serotype 1 strain (2.04%), 2 strains of serum uncertainty type 2 (4.08%). The distribution characteristics of virulence gene were as follows: ail cystoma B yadA virus F accounted for 73.46%, and 36% 49% yad A virus F- accounted for 10.20% of virus F-, and other virulence genotypes accounted for 16.33% / 49%. Conclusion Yersinia enterocolitica carried by pigs in Xining City is mainly pathogenic, and the monitoring of Yersinia enterocolitica in local pigs should be strengthened.
【作者单位】：青海省疾病预防控制中心;中国疾病预防控制中心传染病预防控制所;
【基金】：国家"十二五"传染病科技重大专项实验室监测技术平台项目(No.2012ZX10004-201-002)
【分类号】：R378

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