改构型酸性成纤维细胞生长因子对体外培养肝细胞增殖活性的影响

发布时间：2018-05-16 22:02

本文选题：改构型酸性成纤维细胞生长因子 + 肝细胞　；参考：《安徽医科大学学报》2017年11期

【摘要】：目的探讨改构型酸性成纤维细胞生长因子(MaFGF)对体外培养大鼠肝细胞增殖的影响。方法采用"0.25%胰酶+0.1%胶原酶"消化法分离培养大鼠肝细胞并传代纯化扩增,取第3代细胞鉴定后实验:(1)形态学法观察在不同浓度MaFGF组中细胞的形态变化;(2)将实验随机分为对照组和MaFGF组,MaFGF组加入一系列浓度的MaFGF,MTT法测定不同浓度MaFGF组作用于肝细胞24、48、72h的促增殖活性;(3)MTT法筛选出最适的MaFGF浓度,用此浓度和不加生长因子2种方式培养细胞,并绘制细胞生长曲线。结果(1)MaFGF对体外培养的肝细胞有一定的促增殖作用,但是与浓度不成正比;且实验表明24h的增殖率较其它时间点显著增高(P0.05);比较对照组和各组(4.68×10~(-3)~7.80×10~(-3)mg/LMaFGF组)均差异有统计学意义(P0.05),但各组间对肝细胞的促增殖作用的差异无统计学意义;(2)最适浓度(6.24×10~(-3)mg/L)MaFGF的第4天细胞数量较对照组明显增加(P0.05);MaFGF组的第10天细胞数(12.4×10~4)是对照组(6×10~4)的2.1倍(P0.05)。结论在适宜浓度和时间内,MaFGF对肝细胞具有一定的促增殖作用且不具有浓度依赖性。
[Abstract]:Objective to investigate the effect of modified acidic fibroblast growth factor MaFGF on the proliferation of rat hepatocytes in vitro. Methods Rat hepatocytes were isolated and cultured by "0.25% trypsin 0.1% collagenase" digestion method. The morphological changes of cells in different concentration MaFGF groups were observed by morphological method. The cells were randomly divided into two groups: control group and MaFGF group. The optimal concentration of MaFGF was obtained by MTT assay at 24 ~ 48 ~ (8) h after 72h. The cells were cultured with this concentration and without growth factor, and the cell growth curve was drawn. Results MaFGF could promote the proliferation of hepatocytes in vitro, but it was not proportional to the concentration. The results showed that the proliferation rate at 24 h was significantly higher than that at other time points (P 0.05), and the difference between the control group and the control group was significant (P 0.05), but there was no significant difference between the two groups in promoting the proliferation of hepatocytes. The number of cells on the 4th day of 6. 24 脳 10~(-3)mg/L)MaFGF was significantly higher than that of the control group. The number of cells on the 10th day of P0. 05 10~(-3)mg/L)MaFGF group was 12.4 脳 10 ~ 4), which was 2. 1 times as much as that of the control group (6 脳 10 ~ (4). Conclusion MaFGF can promote the proliferation of hepatocytes in a dose-dependent manner within the appropriate concentration and time.
【作者单位】：广西科技大学医学院健康评估教研室;广西医科大学生命科学研究院;
【基金】：2017年度广西高校中青年教师基础能力提升项目(编号:2017KY0367) 2017年广西科技大学自然科学培育计划项目 2016年度广西科技大学科学基金项目(编号:校科自174514) 2016年广西科技大学科学基金项目培育专项基金
【分类号】：R329.2

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