百日咳杆菌气雾感染小鼠模型的建立

发布时间：2018-05-17 04:26

本文选题：小鼠模型 + 百日咳杆菌　；参考：《医学研究生学报》2017年08期

【摘要】：目的百日咳感染动物模型的建立在以百白破为基础的联合疫苗的研究中具有重要意义。文中探讨不同百日咳攻击菌浓度和不同攻毒时间对BALB/c小鼠的气雾感染效果。方法将BALB/c雌鼠按随机数字表法分为低浓度长时间组、中浓度短时间组、中浓度长时间组、高浓度长时间组和阴性对照组,每组10只。气雾方式攻击小鼠,109cfu/m L攻毒30min(低浓度长时间组)、1010cfu/m L攻毒15 min(中浓度短时间组)、1010cfu/m L攻毒30 min(中浓度长时间组)、1011cfu/m L攻毒30 min(高浓度长时间组),阴性对照组用等渗盐水气雾攻击30 min。分别于攻毒后0、3、7、14、21 d采血测白细胞数和分离肺组织匀浆培养,计算百日咳杆菌肺部克隆数。结果百日咳杆菌气雾攻击小鼠后第3天,与阴性对照组比较,高浓度长时间组白细胞数明显增长(P0.05);第7天,与阴性对照组比较,中浓度长时间组、高浓度长时间组白细胞数(1.74×1010个/L、5×1010个/L)均明显升高(P0.01);第14天,与阴性对照组比较,4个实验组均明显升高(P0.05),其中中浓度短时间组和低浓度长时间组到达峰值(1.77×1010、1.27×1010个/L);第21天,与阴性对照组比较,4个实验组白细胞数均下降,其中高浓度长时间组差异有统计学意义(P0.05)。在攻毒后第3天,通过各组小鼠肺组织匀浆培养均可证实有少量百日咳杆菌存在;第7天和第14天,4个实验组小鼠肺部菌落数均明显升高(P0.05);第21天,4个实验组肺部菌落数均下降。结论该气雾攻击方法可成功建立百日咳杆菌感染小鼠模型。
[Abstract]:Objective to establish an animal model of pertussis infection is of great significance in the study of combined vaccine based on pertussis. The effects of different concentration and time of pertussis attack on aerosol infection in BALB/c mice were studied. Methods female BALB/c rats were randomly divided into low concentration and long time group, middle concentration short time group, medium concentration long time group, high concentration long time group and negative control group, with 10 rats in each group. The mice were attacked by aerosol for 30 mins (1010cfur / mL for 15 mins in the low concentration and long time group (1010cfu-mL for 30 mins in the medium concentration short time group) (1011cfu-mL for 30 mins in the medium concentration long time group and 30 mins in the control group treated with isosmotic saline aerosol for 30 mins). The white blood cell count and lung tissue homogenate culture were collected on 21 days after drug attack, and the lung clones of B. pertussis were calculated. Results compared with the negative control group, the white blood cell count increased significantly in the high concentration long time group on the 3rd day after aerosol attack by pertussis bacillus pertussis, and in the middle concentration long time group on the 7th day, compared with the negative control group. On the 14th day, compared with the negative control group, the white blood cell count in the four experimental groups increased significantly, and the peak value was 1.77 脳 10 ~ (10) / 1.27 脳 1010 / L / L in the short concentration group and the low concentration and long time group respectively, and on the 21st day, there was a significant increase in the white blood cell count of 1.77 脳 10 ~ (10) / L ~ (-1 / L) / L ~ (-1) / L ~ (-1 / L). Compared with the negative control group, the number of white blood cells in the four experimental groups were all decreased, among which there was a significant difference in the high concentration group for a long time (P 0.05). On the 3rd day after the attack, a small amount of pertussis bacillus was confirmed in the lung tissue homogenate culture of mice in each group. On the 7th and 14th days, the number of lung colonies increased significantly in the four experimental groups, and decreased in the four experimental groups on the 21st day. Conclusion the model of mice infected with Bacillus pertussis can be successfully established by this aerosol attack method.
【作者单位】：中国医学科学院/北京协和医学院医学生物学研究所;
【基金】：国家科技重大专项课题(2015ZX09101031)
【分类号】：R-332;R516.6

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