全真一气汤及其多糖对COPD模型小鼠肺组织氧化应激的影响

发布时间：2018-05-17 19:02

本文选题：全真一气汤 + 多糖　；参考：《时珍国医国药》2017年08期

【摘要】：目的观察全真一气汤及其多糖对慢性阻塞性肺疾病(COPD)模型小鼠肺组织氧化应激的影响,从氧化/抗氧化角度探讨全真一气汤及其多糖对COPD疾病的干预效应。方法采用气管内注入脂多糖(LPS)联合熏烟的造模方法建立COPD小鼠模型,随机分为空白组、模型组、全真一气汤低剂量组(QZYQL+CSE)4.3g/kg、全真一气汤高剂量组(QZYQH+CSE)8.6g/kg,全真一气汤多糖组(QZYQL+CSE)1.125g/kg,氨茶碱组(Amino)2.3mg/kg,空白组、模型组灌胃生理盐水0.4ml,各给药组灌胃相应药物,连续16周。检测肺组织中谷胱甘肽过氧化酶(GSH-Px)活性、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量;蛋白印迹法(Western blot)检测肺组织中细胞外调节激酶(ERK1/2)、蛋白激酶B(Akt)、丝裂原活化蛋白激酶P38MAPK(P38)磷酸化水平及NF-E2相关因子(Nrf2)核转移情况。结果与空白组相比,模型组小鼠MDA含量增加,GSH-Px、SOD活性降低;ERK1/2、Akt、P38、Nrf2表达量均升高。与模型组相比,全真一气汤低、高剂量组和多糖组GSH-Px、SOD活性升高,MDA含量减少;ERK1/2、Akt、P38、Nrf2表达量均降低。结论多糖是全真一气汤作用于COPD氧化应激失衡的有效成分,全真一气汤及其多糖对COPD有抗氧化作用,其机制可能是提高GSH-Px、SOD活性,降低MDA含量,通过Nrf2-Akt-P38/ERK1/2通路调节氧化/抗氧化失衡,保护肺组织结构和功能。
[Abstract]:Objective to observe the effect of Quanzhen Yiqi decoction and its polysaccharides on oxidative stress in lung tissue of chronic obstructive pulmonary disease (COPD) mice, and to explore the effect of Quanzhen Yiqi decoction and its polysaccharide on COPD disease. Methods the model of COPD mice was established by intratracheal injection of lipopolysaccharide (LPS) and fumigating smoke. The mice were randomly divided into blank group and model group. QZYQL CSE 4.3 g / kg, QZYQH CSE 8.6 g / kg, QZYQL CSE 1.125 g / kg, aminophylline group Amino 2.3 mg / kg, blank group, model group 0.4 ml normal saline, the corresponding drugs were given to each group for 16 weeks. The activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and malondialdehyde (MDA) were measured. Western blots were used to detect the phosphorylation levels of extracellular regulated kinase (ERK) 1 / 2, protein kinase (BK) Akt1, mitogen-activated protein kinase (P38 MAPK) P38 and nuclear metastasis of NF-E2 related factor (Nrf2) in lung tissue. Results compared with the control group, the MDA content in the model group increased and the activity of GSH-PxX SOD decreased. Compared with the model group, Quanzhen Yiqi decoction was lower, and the activity of GSH-PxX SOD in high dose group and polysaccharide group was increased and MDA content was decreased. Conclusion Polysaccharide is an effective component of Quanzhen Yiqi decoction acting on COPD oxidative stress imbalance. Quanzhen Yiqi decoction and its polysaccharides have antioxidant effect on COPD. The mechanism may be to increase the activity of GSH-PxX SOD and decrease the content of MDA. Nrf2-Akt-P38/ERK1/2 pathway regulates oxidation / antioxidation imbalance and protects lung tissue structure and function.
【作者单位】：广州医科大学研究生院;广州医科大学附属第一医院;广州中医药大学附属第二医院;
【基金】：国家自然科学基金(No.81202674)
【分类号】：R285.5;R-332

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