胡椒碱对胰岛素抵抗综合征模型大鼠脂代谢及作用机制的研究

发布时间：2018-05-18 22:41

本文选题：胡椒碱 + 胰岛素抵抗综合征　；参考：《中国药理学通报》2016年12期

【摘要】：目的探讨胡椒碱(PIP)对胰岛素抵抗综合征(IRS)大鼠甘油三酯(TG)、游离脂肪酸(FFA)、肿瘤坏死因子(TNF-α)、瘦素(Leptin)的作用。方法采用高脂高糖高盐饲料喂养制备IRS大鼠模型。每4周观察各组空腹TG,第4周开始给药,连续给药8周后,分别采用GPO-PAP法、比色法检测TG和FFA水平,ELISA法检测TNF-α、Leptin水平。结果给药8周后,模型大鼠血清TG、FFA、TNF-α、Leptin水平明显升高,不同剂量PIP使TG、FFA、TNF-α、Leptin水平下降。结论 PIP明显改善IRS模型大鼠的脂代谢异常情况,能降低IRS模型大鼠TG和FFA水平,其作用机制可能与下调细胞因子TNF-α、Leptin有关。
[Abstract]:Objective to investigate the effects of piperine (PIP) on triglyceride (TGN), free fatty acid (FFAA), tumor necrosis factor TNF- 伪 (TNF- 伪), leptin (Leptin) in rats with insulin resistance syndrome (IRS). Methods IRS rats were fed with high fat, high sugar and high salt diet. Every 4 weeks, the fasting serum levels of TG and FFA were measured by GPO-PAP method, TG and FFA levels were detected by Elisa and TNF- 伪 -Leptin levels were detected by Elisa. After 8 weeks of continuous administration, TNF- 伪 leptin levels were detected by GPO-PAP, TG and FFA levels respectively. Results after 8 weeks of administration, serum TNF- 伪 leptin levels in the model rats were significantly increased, and the levels of TNF- 伪 leptin were decreased by different doses of PIP. Conclusion PIP can obviously improve the abnormal lipid metabolism in IRS model rats and decrease the levels of TG and FFA in IRS rats. The mechanism may be related to the down-regulation of the cytokine TNF- 伪 leptin.
【作者单位】：云南省中医中药研究院中心实验室;云南大学化学科学与工程学院.药学院自然资源药物化学教育部重点实验室;云南省中医医院中心实验室;云南中烟工业有限公司云南省烟草化学重点实验室;云南中医学院国家中医药管理局中药药理(免疫)三级实验室;
【基金】：国家自然科学基金资助项目(No 81260671)
【分类号】：R285.5;R-332

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