神经生长因子与骨粉混合物对骨缺损愈合的影响

发布时间：2018-05-19 13:00

本文选题：神经生长因子 + Bio-Oss骨粉　；参考：《山西医科大学》2012年硕士论文

【摘要】：目的本实验通过建立大鼠胫骨洞形骨缺损的动物模型，观察NGF/Bio-Oss骨粉混合物是否具有促进成骨的作用。方法 48只雄性SD大鼠，，平均体重220-250g，采用完全随机法分成3组，每组16只。在左侧胫骨结节下1cm处制备约3mm长深达髓腔的骨块缺损模型，实验组：骨缺损植入NGF/Bio-Oss骨粉混合物，对照组：骨缺损植入生理盐水/Bio-Oss骨粉，空白组：为单纯胫骨骨缺损，未做任何处理。每组分别于术后4周和8周各处死动物8只。通过大体解剖标本、X线片、组织形态学及BMP-2免疫组组织化学染色等方法，观察各组各时期新骨生成的情况。结果 1.术后4周大体解剖观察：实验组骨缺损愈合较早，新骨的形成明显比对照组和空白组快；组织学观察结果：实验组新生骨小梁、成骨细胞、新生血管均多于对照组和空白组；BMP-2免疫组化染色结果：染色阳性主要表达在骨膜下和骨小梁周围，阳性细胞主要是一些成骨细胞和未成熟的骨细胞。实验组阳性细胞的平均光密度值明显高于对照组，差别有统计学意义(P0.01)，空白组阳性细胞的平均光密度值最低，与其他组相比，差别有统计学意义(P0.01)。 2.术后8周大体解剖观察：各组骨缺损基本愈合，其中实验组生成的新骨与周围骨组织一致；X线片观察结果：实验组和对照组骨缺损区影像的密度接近周围骨组织，轮廓与原骨缺损区一致，空白组骨缺损区影像密度稍低，有骨缺损切迹；组织学观察结果：各组的骨缺损区均为新生骨充填，空白组骨缺损区中央的骨质成熟度最低。结论 NGF/Bio-Oss骨粉混合物能明显促进大鼠胫骨洞形骨缺损早期新骨的形成及骨量的增加，促进骨质的成熟，缩短愈合时间。
[Abstract]:Purpose In this study, we established an animal model of rat tibial cavitation bone defect and observed whether the mixture of NGF/Bio-Oss bone powder could promote osteogenesis. Method 48 male SD rats with an average weight of 220-250 g were randomly divided into 3 groups with 16 rats in each group. The model of bone mass defect was made at 1cm under left tibial nodule. Experimental group: bone defect was implanted with NGF/Bio-Oss bone powder mixture; control group: bone defect implanted physiological saline / Bio-Oss bone powder; blank group: simple tibial bone defect. No handling has been done. In each group, 8 animals died 4 and 8 weeks after operation. The new bone formation in each group was observed by the methods of gross anatomical specimen X-ray, histomorphology and BMP-2 immunohistochemical staining. Result 1. 4 weeks after operation Gross anatomy observation: the bone defect healed earlier in the experimental group, and the formation of new bone was faster than that in the control group and blank group. The results of immunohistochemical staining of BMP-2 in neovascularization group and control group were as follows: the positive staining was mainly located under periosteum and around trabecular bone, and the positive cells were mainly osteoblasts and immature bone cells. The average optical density of the positive cells in the experimental group was significantly higher than that in the control group, the difference was statistically significant (P 0.01), and the average optical density of the positive cells in the blank group was the lowest, compared with other groups, the difference was statistically significant. 2. 8 weeks after operation Gross anatomy observation: the bone defect was healed in each group, and the results of X-ray film showed that the density of the bone defect area in the experimental group and the control group was close to the surrounding bone tissue, and the density of the new bone was the same as that of the surrounding bone tissue. The outline was consistent with the original bone defect area, the image density of the bone defect area in the blank group was slightly lower, and there was a bone defect notch. The histological observation showed that the bone defect area in each group was filled with new bone, and the bone maturity in the center of the bone defect area in the blank group was the lowest. Conclusion NGF/Bio-Oss bone powder mixture could significantly promote the formation of new bone and increase of bone mass in the early stage of tibial cave-shaped bone defect in rats, promote bone maturation and shorten the healing time.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R687.3;R-332

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