非洲绿猴血液学和血清生化指标的测定及分析

发布时间：2018-05-21 10:52

  本文选题：非洲绿猴 + 血液学指标　； 参考：《中国比较医学杂志》2017年11期

【摘要】：目的检测50只健康非洲绿猴血液学、血清生化指标,分析不同性别、不同年龄段(青幼年组:1~3岁,成年组:4~6岁)非洲绿猴的血液学、血清生化指标的差异。方法应用全自动血细胞分析仪及血液生化分析仪分别测定清醒状态下健康非洲绿猴血液学及血清生化指标。结果血液学指标中青幼年组与成年组差异显著的指标有红细胞数(RBC)、血红蛋白(HGB)、血细胞压积(HCT)、中性粒细胞百分比(NEUT%)、淋巴细胞百分比(LYMPH%)、单核粒细胞百分比(MONO%)、嗜碱性粒细胞百分比(BASO%)(P0.05)。青幼年组中雌猴与雄猴差异显著的指标有白细胞数(WBC)、RBC、HGB、HCT、NEUT%、LYMPH%、MONO%(P0.05),成年组中雌猴与雄猴差异显著的指标有HCT(P0.05),其它指标差异不显著。血清生化指标中青幼年组与成年组差异显著的指标有白蛋白(ALB)、天门冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)、肌酐(CREA)(P0.05),青幼年组雌猴与雄猴差异显著的指标有CREA(P0.05),成年组雌猴与雄猴差异显著的指标有总蛋白(TP)、胆固醇(CHOL)(P0.05),其它指标差异不显著。结论本文初步建立了非洲绿猴的常规生物学数据,为评价其生物学特性及相关应用提供一定的数据基础,可供参考。
[Abstract]:Objective to detect the hematology and serum biochemical indexes of 50 healthy African green monkeys, and to analyze the differences of hematology and serum biochemical indexes of African green monkeys of different sex and age (young and young group: 1 + 3 years old, adult group: 4 ~ 6 years old). Methods Hematology and serum biochemical indexes of healthy African green monkey were measured by automatic hematology analyzer and blood biochemistry analyzer respectively. Results there were significant differences in hematologic indexes between young and green groups, such as red blood cell count (RBC), hemoglobin (HGB), hematocrit (HCT), neutrophil percentage (neuto), lymphocyte percentage (LYMPH), mononuclear cell (mononuclear), basic granulocyte (basophil) and basophil (basic granulocyte). The significant differences between female and male monkeys were as follows: the white blood cell count (WBC) of RBC / HGBHGBHGBHGBHGBHGBHGBHGBRBBCNBBBCHGBHGBHGBHGBHBNBBCNBBBCHGB / HGBHGB / HGBHGB / HGB / HGBHGB / HGBC / HGBC / HGBB / HGBC / HGB / HGB / HGB / HGB / HGBHGB / HGBHGB@@ Serum biochemical indexes: Albumin ALBX, aspartate aminotransferase (ASTT), alkaline phosphatase (ALPN), creatinine (CREAEAA) P0.05 in serum, creatine in young and young females and adult monkeys, and adult female monkeys, respectively. There were significant differences in total protein TPN and cholesterol Choll (P0.05) between male and male monkeys, but there was no significant difference in other indexes. Conclusion the conventional biological data of African green monkey are established in this paper, which can be used as a reference for the evaluation of its biological characteristics and related applications.
【作者单位】： 军事医学科学院实验动物中心;
【基金】：国家自然科学基金(编号:31272514,31501908)
【分类号】：R-33
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本文编号：1918902