大鼠肾缺血—再灌注损伤后AQP2的表达及七氟醚预处理对肾保护作用的探讨

发布时间：2018-05-23 13:20

本文选题：AQP2 + 七氟醚　；参考：《吉林大学》2012年硕士论文

【摘要】：背景和目的：肾脏缺血-再灌注损伤（ischemia reperfusion,IRI）是临床医疗中常见的病理生理过程，急性肾小管坏死（ATN）是该过程中重要和关键的环节。AQP2是水通道蛋白家族(AQPS)中的一种，位于肾脏集合管主细胞管腔侧和靠管腔侧的囊泡内~[1,2]，是调节肾脏集合管对水通透性的主要水通道蛋白，在肾脏缺血-再灌注损伤后12-48小时表达显著减低~[3~4]。七氟醚对心~[5~7]、脑~[8-10]、肝~[11]、肺~[12,13]等脏器和组织I/R损伤都有不同程度的保护作用已经被证实，在学术界七氟醚预处理对肾脏缺血-再灌注是否具有保护作用仍未成定论，本实验诣探讨大鼠肾缺血-再灌注后AQP2的表达和吸入麻醉剂七氟醚预处理对急性肾缺血再灌注损伤的保护作用。方法：SD大鼠随机分为假手术组（B）、对照组(I/R组)和七氟醚预处理组（Pre-S），常温下建立大鼠急性肾缺血再灌注损伤模型,缺血再灌注后24h用生化分析仪分别检测血清尿素氮(BUN)、肌酐(Cr)，用相应试剂盒检测超氧化物歧化酶(SOD)、丙二醛(MDA)；采集术前和术后24小时尿样，，检测尿量、尿比重；采用Real Time RT-PCR及免疫组织化学法测定肾髓质AQP2mRNA及蛋白表达情况，观察肾组织的病理学变化。探讨七氟醚于缺血前、后处理肾脏损伤程度。结果：与B组比较,其它两组血清BUN、Cr，及MDA水平显著增加(P0.05), Pre-S组BUN、Cr、MDA低于I/R组(P 0.05)。与B组比较,其它两组SOD明显下降，与I/R组比较, Pre-S组SOD升高。与B组比较，其它两组尿量增加，尿比重降低，Pre-S组尿量低于I/R组(P0.05)，尿比重高于I/R组。光学显微镜下I/R组肾小管结构破坏严重，间质充血，水肿明显，有大量炎细胞侵润，Pre-S组结构仅见部分肾小管上皮细胞轻度肿胀,轻微变性,无明显坏死,肾间质水肿，充血，炎性细胞浸润不明显，Pre-S组肾组织病理损伤分级明显低于I/R组(P0.05)。肾髓质AQP2mRNA及蛋白表达下降，Pre-S组较I/R组下降轻微(P0.05)。结论：（1）AQP2在肾脏缺血45min,再灌注损伤24小时后表达显著减低。（2）七氟醚预处理对大鼠急性肾缺血再灌注损伤具有保护作用。（3）七氟醚上调肾脏缺血-再灌注损伤后AQP2的表达。
[Abstract]:Background & objective: renal ischemia-reperfusion injury (IRI) is a common pathophysiological process in clinical medicine. Acute tubular necrosis (ATN) is an important and key link in this process. AQP2 is one of aquaporin family AQPSs. Located in the lumen side of the main cell of the renal collecting duct and the side of the lumen, it is the main aquaporin that regulates the water permeability of the renal collecting duct, and the expression of ~ (34) is significantly decreased 12-48 hours after the renal ischemia-reperfusion injury. The protective effects of sevoflurane on I / R damage in organs and tissues such as heart, brain, liver, lung and so on have been proved to be of different degrees. Whether sevoflurane preconditioning has protective effect on renal ischemia-reperfusion in academic circles has not been concluded. The purpose of this study was to investigate the expression of AQP2 and the protective effect of sevoflurane preconditioning on acute renal ischemia-reperfusion injury in rats. Methods Twenty SD rats were randomly divided into sham-operation group (control group) and sevoflurane preconditioning group (Pre-Sn). Acute renal ischemia-reperfusion injury model was established at room temperature in rats. The serum urea nitrogen bun, creatinine, superoxide dismutase (SOD), malondialdehyde (MDA) MDAA were detected by biochemical analyzer 24 hours after ischemia and reperfusion, and urine volume and specific gravity of urine were measured 24 hours before and 24 hours after reperfusion. The expression of AQP2mRNA and protein in renal medulla was determined by Real Time RT-PCR and immunohistochemistry, and the pathological changes of renal tissue were observed. To investigate the degree of renal injury after sevoflurane treatment before and after ischemia. Results: compared with group B, the serum levels of BUNC and MDA in the other two groups were significantly higher than those in group B (P 0.05), and the levels of Pre-S in group B were lower than those in group I / R (P 0.05). Compared with group B, SOD in the other two groups was significantly decreased, and SOD in Pre-S group was higher than that in group I / R. Compared with group B, the urine volume of the other two groups was increased, the urine specific gravity of Pre-S group was lower than that of I / R group (P 0.05), and the urine specific gravity was higher than that of I / R group. In the I / R group, the tubule structure was seriously damaged, interstitial hyperemia and edema were observed under optical microscope, and a large number of inflammatory cells infiltrated the pre-S group. Only some tubule epithelial cells were slightly swollen, slightly degenerated, no obvious necrosis, edema, hyperemia, and hyperemia were observed in the I / R group. The pathological grade of renal tissue in Pre-S group was significantly lower than that in I / R group (P 0.05). The expression of AQP2mRNA and protein in renal medulla decreased slightly in Pre-S group than in I / R group (P 0.05). Conclusion: The expression of AQP2 was significantly decreased after renal ischemia for 45 min and reperfusion injury for 24 hours. 2) sevoflurane preconditioning has protective effect on acute renal ischemia reperfusion injury in rats. Sevoflurane upregulated the expression of AQP2 after renal ischemia-reperfusion injury.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R363

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