肾脑复元汤联合人脐带间充质干细胞移植对大脑中动脉缺血再灌注模型大鼠炎症因子的影响

发布时间：2018-05-23 20:38

本文选题：缺血性中风 + 脑缺血再灌注　；参考：《中医杂志》2017年14期

【摘要】：目的探究肾脑复元汤联合人脐带间充质干细胞(h UC-MSCs)移植治疗缺血性中风的可能机制。方法将75只大鼠随机分为假手术组、模型组、中药组、干细胞组、联合组各15只,除假手术组外其余各组建立大脑中动脉缺血再灌注模型,造模后第1天中药组和联合组给予肾脑复元汤11.6 g/kg灌胃,假手术组、模型组、干细胞组予10 ml/kg蒸馏水灌胃,每天1次,共15天;干细胞组和联合组造模后第1天尾静脉缓慢注射1×106个h UC-MSCs。分别于术前24 h、术后2 h及术后第4、8、15天进行神经功能评分;分别于第4、8、15天时采用TUNEL染色标记凋亡细胞,检测血清肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)、白细胞介素6(IL-6)、白细胞介素10(IL-10)的表达。结果与模型组相比,中药组(第8、15天)、干细胞组及联合组(第4、8、15天)大鼠神经功能评分降低,凋亡细胞数减少,IL-1β、IL-6表达降低,IL-10表达升高(P0.05或P0.01);与中药组比较,联合组第4、8天TNF-α表达降低(P0.05);与中药组及干细胞组比较,联合组第8天时IL-1β、IL-6表达降低,第8、15天时神经功能评分降低、凋亡细胞数减少、IL-10表达升高(P0.05)。结论肾脑复元汤联合h UC-MSCs移植可促进大脑中动脉缺血再灌注大鼠神经功能修复,其机制可能与调节炎症反应有关。
[Abstract]:Objective to explore the possible mechanism of Shennao Fuyuan decoction combined with human umbilical cord mesenchymal stem cell transplantation for ischemic stroke. Methods Seventy-five rats were randomly divided into sham-operation group, model group, traditional Chinese medicine group, stem cell group and combination group. On the first day after modeling, the traditional Chinese medicine group and the combined group were given 11.6 g/kg Shennao Fuyuan decoction, while the sham operation group, model group and stem cell group were given 10 ml/kg distilled water once a day for 15 days. In the stem cell group and the combined group, UC-MSCs was injected slowly at 1 脳 106 h on the first day after modeling. Nerve function scores were assessed at 24 hours before operation, 2 hours after operation and 8 days after operation, and apoptotic cells were labeled with TUNEL staining on day 4, 8 and 15, respectively. The expression of TNF- 伪, IL-1 尾, IL-6 and IL-10 in serum were detected. Results compared with the model group, the scores of nerve function and the number of apoptotic cells decreased in the traditional Chinese medicine group (815 days), stem cell group and combination group (4th day 815 days), the number of apoptotic cells decreased and the expression of IL-10 increased (P0.05 or P0.01), compared with the traditional Chinese medicine group. Compared with the traditional Chinese medicine group and stem cell group, the expression of IL-1 尾 -IL-6 in the combined group was decreased on the 8th day, the neurological function score was decreased on the 8th day, and the number of apoptotic cells was decreased, and the expression of IL-10 was increased in the combined group. Conclusion Shennao Fuyuan decoction combined with h UC-MSCs transplantation can promote the repair of nerve function in rats with middle cerebral artery ischemia reperfusion, and its mechanism may be related to the regulation of inflammation.
【作者单位】：中南大学湘雅三医院;湖南中医药大学第一附属医院;
【基金】：国家自然科学基金(81273751,81573941)
【分类号】：R285.5;R-332

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